机构：[1]Henan Provincial Key Laboratory of Children’s Genetics and Metabolic Diseases, Children’s Hospital Affiliated of Zhengzhou University, Henan Children’s Hospital, Zhengzhou Children’s Hospital, Zhengzhou 450018, China[2]Department of Clinical Laboratory, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300000, China[3]Department of Laboratory Medicine, Guangdong Hospital of Traditional Chinese Medicine, Zhuhai 510120, China大德路总院珠海院区检验科大德路总院检验科广东省中医院
The abnormal change of thrombin concentration is closely related with various diseases. By using proximity binding-induced DNA network assembled by nonlinear hybridization chain reaction (HCR), we here developed an enzyme-free and ultrasensitive electrochemical strategy for analyzing thrombin in human serums. The binding of the two different aptamers to a thrombin enhanced the local concentration of the aptamers and facilitates the formation of an intact strand. Subsequently, the formed strand caused nonlinear HCR, forming DNA network. The DNA network bound to the capture probes (CP)-modified electrode, producing electrochemical signal of MB. Benefiting from the proximity binding-induced DNA network, the proposed biosensor exhibited a wide linear range from 1.0 pM to 1.0 nM with a low detection limit of 0.56 pM and high specificity. More importantly, the developed enzyme-free electrochemical biosensor has been used to detect thrombin in serum samples with good performance. This makes such a strategy possess potential promise in the application of clinical diagnosis.
基金：
"new development project" of the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine [201905]
第一作者机构：[1]Henan Provincial Key Laboratory of Children’s Genetics and Metabolic Diseases, Children’s Hospital Affiliated of Zhengzhou University, Henan Children’s Hospital, Zhengzhou Children’s Hospital, Zhengzhou 450018, China
通讯作者：
推荐引用方式(GB/T 7714)：
Li Wei,Zhao Ding,Tian Dongdong,et al.Electrochemical aptasensor based on proximity binding-induced DNA networked for enzyme-free and ultrasensitive detection of thrombin[J].JOURNAL OF ELECTROANALYTICAL CHEMISTRY.2021,895:doi:10.1016/j.jelechem.2021.115447.
APA：
Li, Wei,Zhao, Ding,Tian, Dongdong,Zhai, Manman,Xu, Hongdan...&Sang, Ye.(2021).Electrochemical aptasensor based on proximity binding-induced DNA networked for enzyme-free and ultrasensitive detection of thrombin.JOURNAL OF ELECTROANALYTICAL CHEMISTRY,895,
MLA：
Li, Wei,et al."Electrochemical aptasensor based on proximity binding-induced DNA networked for enzyme-free and ultrasensitive detection of thrombin".JOURNAL OF ELECTROANALYTICAL CHEMISTRY 895.(2021)
