机构：[1]Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 511436, China.[2]Guangdong Medical Innovation Platform for Translation of 3D Printing Application, The Third Affiliated Hospital of Southern Medical University, Southern Medical University, Guangzhou 510630, China.[3]Department of Stomatology, Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou 510095, China.[4]Department of Anatomy, Guangdong Provincial Key Laboratory of Digital Medicine and Biomechanics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.[5]Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510799, China.[6]Guangxi Key Laboratory of Birth Defects Research and Prevention, Nanning 530005, China.
Non-essential proteins for viral replication affect host cell metabolism, while the function of the UL43 protein of herpes simplex virus 1 (HSV-1) is not clear. Herein, we performed a comprehensive microarray analysis of HUVEC cells infected with HSV-1 and its UL43-deficient mutant and found significant variation in genes associated with cellular energy metabolic pathways. The localization of UL43 protein in host cells and how it affects cellular energy metabolism pathways were further investigated. Internalization analysis showed that the UL43 protein could be endocytosis-mediated by YPLF motif (aa144-147) and localized to mitochondria. At the same time, more ATP was produced by coupling with mitochondrial small G protein ARF-like 2 (ARL2) GTPase, which triggered the phosphorylation of ANT1 (SLC25A4) to affect the opening degree of mitochondrial permeability transition pore (mPTP), and significantly promoted the aerobic oxidation and oxidative phosphorylation of glucose. Our study shows that UL43 mediates the improvement of host cell metabolism after HSV-1 infection. Additionally, UL43 protein could be a valuable ATP-stimulating factor for mammalian cells.
基金：
This work was supported by National Natural Science Foundation of China (82073747), and
Guangdong Basic and Applied Basic Research Foundation (2021A1515111074), and the fellowship
of China Postdoctoral Science Foundation (2021M701620), Yingcai Project in Guangzhou Development
District (2019 Year), Scientific research project of Traditional Chinese Medicine Bureau of
Guangdong Province (20232007), President Foundation of The Third Affiliated Hospital of Southern
Medical University (YQ2021001), Guangxi Key Laboratory of Birth Defects Research and Prevention
(GXWCHZDKF-2022-21).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|3 区生物学
小类|3 区细胞生物学
最新[2025]版：
大类|2 区生物学
小类|3 区细胞生物学
第一作者：
第一作者机构：[1]Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 511436, China.
共同第一作者：
通讯作者：
通讯机构：[1]Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 511436, China.[2]Guangdong Medical Innovation Platform for Translation of 3D Printing Application, The Third Affiliated Hospital of Southern Medical University, Southern Medical University, Guangzhou 510630, China.[4]Department of Anatomy, Guangdong Provincial Key Laboratory of Digital Medicine and Biomechanics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.[6]Guangxi Key Laboratory of Birth Defects Research and Prevention, Nanning 530005, China.
推荐引用方式(GB/T 7714)：
Deng Jianshan,Zhong Zhiying,Geng Chengxu,et al.Herpes Simplex Type 1 UL43 Multiple Membrane-Spanning Protein Increases Energy Metabolism in Host Cells through Interacting with ARL2[J].Cells.2022,11(22):doi:10.3390/cells11223594.
APA：
Deng Jianshan,Zhong Zhiying,Geng Chengxu,Dai Zhenning,Zheng Weihan...&Li Shiyu.(2022).Herpes Simplex Type 1 UL43 Multiple Membrane-Spanning Protein Increases Energy Metabolism in Host Cells through Interacting with ARL2.Cells,11,(22)
MLA：
Deng Jianshan,et al."Herpes Simplex Type 1 UL43 Multiple Membrane-Spanning Protein Increases Energy Metabolism in Host Cells through Interacting with ARL2".Cells 11..22(2022)
