Morroniside alleviates lipopolysaccharide-induced inflammatory and oxidative stress in inflammatory bowel disease by inhibiting NLRP3 and NF-κB signaling pathways
机构：[1]Department of Pathology,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,Guangdong,China深圳市康宁医院深圳市人民医院深圳医学信息中心中国医科大学附属盛京医院中国医科大学盛京医院[2]Department of Traditional Chinese Medicine,Shenzhen People’s Hospital Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,Guangdong,China深圳市康宁医院深圳市人民医院深圳医学信息中心中国医科大学附属盛京医院中国医科大学盛京医院[3]Department of Pathology,Shenzhen Second People’s Hospital,Shenzhen,Guangdong,China深圳市康宁医院深圳医学信息中心[4]Department of Radiology,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,Guangdong,China深圳市康宁医院深圳市人民医院深圳医学信息中心中国医科大学附属盛京医院中国医科大学盛京医院
To investigate the effects of morroniside on inflammatory and oxidative stress in lipopolysaccharide (LPS)-induced inflammatory bowel disease (IBD) cell model.NCM460 cells were treated with 2-, 5-, or 10-μg/mL LPS for 24 h to develop an IBD cell model. MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) colorimetric assay was performed to uncover the role of morroniside on the viability of LPS-treated NCM460 cells. Flow cytometry and immunoblot assays were performed to confirm the effects of morroniside on the apoptosis of LPS-treated NCM460 cells. Quantitative polymerase chain reaction and enzyme-linked-immunosorbent serologic assays were performed to confirm the effects of morroniside on inflammatory and oxidative stress by measuring the levels of tumor necrosis factor-α, interleukin-1β, IL-6, superoxide dismutase, malondialdehyde, total antioxidant capacity, and myeloperoxidase. In addition, immunoblot and immunofluorescence assays were performed to detect the effects of morroniside on NLRP3 and NF-κB pathways.Monosine attenuated LPS-induced injury of NCM460 cells. Monosine reduced LPS-induced inflammation in NCM460 cells. In addition, morroniside reduced LPS-induced oxidative stress in NCM460 cells. Mechanically, morroniside suppressed NLRP3 and NF-κB pathways, and alleviated LPS-induced inflammatory and oxidative stress in IBD.Morroniside could serve as a promising drug for treating IBD.
第一作者机构：[1]Department of Pathology,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,Guangdong,China[*1]Department of Pathology,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University),and The First Affiliated Hospital,Southern University of Science and Technology,No. 1017 Dongmen North Road,Luohu District,Shen Zhen,China.
通讯作者：
通讯机构：[1]Department of Pathology,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,Guangdong,China[*1]Department of Pathology,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University),and The First Affiliated Hospital,Southern University of Science and Technology,No. 1017 Dongmen North Road,Luohu District,Shen Zhen,China.
推荐引用方式(GB/T 7714)：
Zhang Shifen,Lai Qiaohua,Liu Liming,et al.Morroniside alleviates lipopolysaccharide-induced inflammatory and oxidative stress in inflammatory bowel disease by inhibiting NLRP3 and NF-κB signaling pathways[J].ALLERGOLOGIA ET IMMUNOPATHOLOGIA.2022,50(6):93-99.doi:10.15586/aei.v50i6.674.
APA：
Zhang Shifen,Lai Qiaohua,Liu Liming,Yang Yajie&Wang Juan.(2022).Morroniside alleviates lipopolysaccharide-induced inflammatory and oxidative stress in inflammatory bowel disease by inhibiting NLRP3 and NF-κB signaling pathways.ALLERGOLOGIA ET IMMUNOPATHOLOGIA,50,(6)
MLA：
Zhang Shifen,et al."Morroniside alleviates lipopolysaccharide-induced inflammatory and oxidative stress in inflammatory bowel disease by inhibiting NLRP3 and NF-κB signaling pathways".ALLERGOLOGIA ET IMMUNOPATHOLOGIA 50..6(2022):93-99
