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ACSF2-mediated ferroptosis is involved in ulcerative colitis

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机构: [1]The Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang, Guangdong 524023, China [2]The Marine Biomedical Research Institute of Guangdong Zhanjiang, Zhanjiang, Guangdong 524023, China [3]The First Clinical College, Guangdong Medical University, Zhanjiang, Guangdong 524023, China [4]Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing Institute of Chinese Medicine, Beijing 100000, China
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关键词: Ferroptosis Machine learning ACSF2 Ulcerative colitis

摘要:
To investigate the role of ferroptosis-related genes in the induction into ulcerative colitis (UC) and provide new strategies for the prevention and treatment of UC.We screened the UC dataset from the GEO database and obtained ferroptosis-related genes from FerrDB and GeneCards. The R package "CancerSubtypes" was performed to identify the UC subtypes, followed by Short Time-series Expression Miner (STEM) analysis. The key genes were further screened by machine learning algorithms (LASSO and SVM-RFE). WB and IHC verified the changes in the expression content of ACSF2 in vivo and in vitro models. The changes in intracellular ROS and Fe2 + levels were detected.Through bioinformatics analysis, we selected the ferroptosis-related gene ACSF2 (acyl CoA synthetase family member 2), which is significantly associated with immune-related pathways "Toll-like receptor signaling pathway", "NF-kappa B signaling pathway" and "NOD-like receptor signaling pathway". The expression of ACSF2 was significantly down-regulated in UC animals, Salmonella typhimurium colitis models and cell models, while the ferroptosis inhibitor Fer-1 reversed the expression of ACSF2 in LPS-induced cell models, indicating that the ferroptosis-related gene ACSF2 plays an important role in mediating ferroptosis and inflammation, and is expected to become a new target for further research.Ferroptosis is closely associated with the development of UC, and the ferroptosis-related gene ACSF2 can be used as a potential biomarker for the diagnosis and treatment of UC.Copyright © 2022. Published by Elsevier Inc.

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出版当年[2021]版:
大类 | 3 区 医学
小类 | 3 区 医学:研究与实验 3 区 药学
最新[2025]版:
大类 | 3 区 医学
小类 | 2 区 药学 3 区 医学:研究与实验
第一作者:
第一作者机构: [1]The Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang, Guangdong 524023, China [2]The Marine Biomedical Research Institute of Guangdong Zhanjiang, Zhanjiang, Guangdong 524023, China [*1]The Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
通讯作者:
通讯机构: [1]The Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang, Guangdong 524023, China [2]The Marine Biomedical Research Institute of Guangdong Zhanjiang, Zhanjiang, Guangdong 524023, China [*1]The Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
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