机构：[1]State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.[2]The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.广东省中医院[3]Guangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou 510632, China.[4]Laboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.深圳市中医院深圳医学信息中心[5]Medical Research Center, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.广东省人民医院
Zika virus (ZIKV) is an RNA-enveloped virus that belongs to the Flavivirus genus, and ZIKV infections potentially induce severe neurodegenerative diseases and impair male fertility. Palmitoylation is an important post-translational modification of proteins that is mediated by a series of DHHC-palmitoyl transferases, which are implicated in various biological processes and viral infections. However, it remains to be investigated whether palmitoylation regulates ZIKV infections. In this study, we initially observed that the inhibition of palmitoylation by 2-bromopalmitate (2-BP) enhanced ZIKV infections, and determined that the envelope protein of ZIKV is palmitoylated at Cys308. ZDHHC11 was identified as the predominant enzyme that interacts with the ZIKV envelope protein and catalyzes its palmitoylation. Notably, ZDHHC11 suppressed ZIKV infections in an enzymatic activity-dependent manner and ZDHHC11 knockdown promoted ZIKV infection. In conclusion, we proposed that the envelope protein of ZIKV undergoes a novel post-translational modification and identified a distinct mechanism in which ZDHHC11 suppresses ZIKV infections via palmitoylation of the ZIKV envelope protein.
基金：
This research was funded by: the National Nature Science Foundation of China, grant
number 81973549 and U22A20335; the Key-Area Research and Development Program of Guangdong
Province, grant number 2020B1111100002; and Guangzhou University of Chinese Medicine First-Class
Universities and Top Disciplines Scientific Research Team Projects, grant number 2021XK16
第一作者机构：[1]State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
共同第一作者：
通讯作者：
通讯机构：[1]State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.[3]Guangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou 510632, China.[4]Laboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
推荐引用方式(GB/T 7714)：
Hu Dingwen,Zou Haimei,Chen Weijie,et al.ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein[J].VIRUSES-BASEL.2023,15(1):doi:10.3390/v15010144.
APA：
Hu Dingwen,Zou Haimei,Chen Weijie,Li Yuting,Luo Ziqing...&Li Geng.(2023).ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein.VIRUSES-BASEL,15,(1)
MLA：
Hu Dingwen,et al."ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein".VIRUSES-BASEL 15..1(2023)
