机构：[1]Guangdong Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, 510632, China[2]Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China[3]Laboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China[4]Department of Pharmacy, First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China深圳市中医院深圳医学信息中心[5]Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, 510060, China[6]The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510000, China[7]Guangzhou University of Chinese Medicine, Guangzhou, 510006, China[8]Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China华中科技大学同济医学院附属同济医院
The emergence and re-emergence of Zika virus (ZIKV), is a cause for international concern. These highly pathogenic arboviruses represent a serious health burden in tropical and subtropical areas worldwide. Despite these burdens, antiviral therapies do not exist, and inhibitors of ZIKV are therefore urgently needed. To elucidate the anti-ZIKV effect of lycorine, we used reverse transcription-quantitative real-time PCR (qRT-PCR), immunofluorescence, (westernwestern) blot, and plaque forming assay to analyse viral RNA (vRNA), viral protein, progeny virus counts, and validated inhibitors in vitro using a variety of cell lines. Additionally, we found that lycorine acts post-infection according to time-of-addition assay, and inhibits RdRp activity. Lycorine protected AG6 mice against ZIKV-induced lethally by decreasing the viral load in the blood. Due to its potency and ability to target ZIKV infection in vivo and in vitro, lycorine might offer promising therapeutic possibilities for combatting ZIKV infections in the future.
基金：
Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2017A030313626]; Excellent Young Scholars Project of the First Affiliated Hospital of Guangzhou University of Chinese Medicine [2019QN22, 2017ZWB10]; Science & Technology Planning Project of Guangzhou [201804010029]; National Nature Science Foundation of ChinaNational Natural Science Foundation of China [81973549, 81803813, 81730061, 81471942]; State Key Laboratory of Natural Medicines [SKLNMKF201906]; Science & Technology Planning Project of Guangdong Province Office of Education [2018KCXTD007, 2014GKXM032]; Program of Guangdong Natural Science Foundation of China [S201230006598]; Key Research Projects of GZUCM First-class Universities and Top Disciplines [A1-AFD018191A17]; GZUCM First-class Universities and Top Disciplines Scientific Research Team Projects [2019KYTD102]; Guangdong Province “Pearl River Talent Plan”Innovation and Entrepreneurship Team Project [2017ZT07Y580]
第一作者机构：[1]Guangdong Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, 510632, China
共同第一作者：
通讯作者：
通讯机构：[1]Guangdong Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, 510632, China[3]Laboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China[*1]Guangdong Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Laboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
推荐引用方式(GB/T 7714)：
Chen Huini,Lao Zizhao,Xu Jiangtao,et al.Antiviral activity of lycorine against Zika virus in vivo and in vitro[J].VIROLOGY.2020,546:88-97.doi:10.1016/j.virol.2020.04.009.
APA：
Chen, Huini,Lao, Zizhao,Xu, Jiangtao,Li, Zhaoxin,Long, Haishan...&Wu, Jianguo.(2020).Antiviral activity of lycorine against Zika virus in vivo and in vitro.VIROLOGY,546,
MLA：
Chen, Huini,et al."Antiviral activity of lycorine against Zika virus in vivo and in vitro".VIROLOGY 546.(2020):88-97
