机构：[1]Gaozhou Peoples Hosp, Dept Oncol, Sect 3, Gaozhou, Peoples R China[2]Guangzhou Univ Chinese Med, Med Coll Acu Moxi & Rehabil, South China Res Ctr Acupuncture & Moxibust, Guangzhou, Peoples R China[3]Guangzhou Univ Chinese Med, Guangdong Prov Hosp Chinese Med, Affiliated Hosp 2, Guangzhou, Peoples R China广东省中医院[4]Southern Med Univ, Affiliated Nanfang Hosp, Branch Tai He Hosp, Guangzhou, Peoples R China
In this study, we investigated the functional role of eukaryotic initiation factor 5B (EIF5B) in hepatocellularcarcinoma (HCC) and the underlying mechanisms. Bioinformatics analysis demonstrated that the EIF5B transcriptand protein levels as well as the EIF5Bcopy number were significantly higher in the HCC tissues compared with thenon-cancerous liver tissues. Down-regulation of EIF5B significantly decreased proliferation and invasiveness of theHCC cells. Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stemcell (CSC) phenotype. Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU).In the HCC cells, activation of the NF-kappa B signaling pathway and IkB phosphorylation was significantly reducedby EIF5B silencing. IGF2BP3 increased the stability of the EIF5B mRNA in an m6A-dependent manner. Our datasuggested that EIF5B is a promising prognostic biomarker and therapeutic target in HCC
基金：
National Natural Science Foundation of China [81773167]; Project of Traditional Chinese Medicine of Guangdong Administration [20132155]; Medical and Health Science and Technology Project of Guangzhou Baiyun District [2020-YL-002]
第一作者机构：[1]Gaozhou Peoples Hosp, Dept Oncol, Sect 3, Gaozhou, Peoples R China
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通讯作者：
推荐引用方式(GB/T 7714)：
LI Xiaoyin,Wang Qian,Liang Hongfeng,et al.IGF2BP3-induced activation of EIF5B contributes to progression ofhepatocellular carcinoma cells br[J].ONCOLOGY RESEARCH.2022,30(2):77-87.doi:10.32604/or.2022.026511.
APA：
LI, Xiaoyin,Wang, Qian,Liang, Hongfeng,Chen, Shisheng,Chen, Haiwen...&Yang, Changfu.(2022).IGF2BP3-induced activation of EIF5B contributes to progression ofhepatocellular carcinoma cells br.ONCOLOGY RESEARCH,30,(2)
MLA：
LI, Xiaoyin,et al."IGF2BP3-induced activation of EIF5B contributes to progression ofhepatocellular carcinoma cells br".ONCOLOGY RESEARCH 30..2(2022):77-87
