机构：[1]Beijing Univ Chinese Med, Sch Life Sci, Beijing 100029, Peoples R China[2]Guangzhou Univ Chinese Med, Clin Coll 2, Guangzhou 510405, Peoples R China广东省中医院[3]Guangzhou Univ Chinese Med, Sch Basic Med Sci, Guangzhou 510405, Peoples R China[4]Guangzhou Univ Chinese Med, Affiliated Hosp 1, Guangzhou 510405, Peoples R China
The targets and mechanisms of Si-Wu-Tang (SWT) against (Breast cancer) BRCA were identified and a survival model and nomogram was construted by network pharmacology, bioinformatic analysis and in vitro experiments. A total of 72 anti-breast cancer SWT targets were selected, among which eleven genes (MAOA,SQLE,CACNA2D1,GLI1,RORB,ITGB3,TACR1, NR3C2,CA3,RBP4 and PTK6) were used to construct a novel prognostic model of breast cancer. The anti-breast cancer activity of SWT was related to the modulation of the receptor tyrosine kinases signaling pathways. Moreover, two compounds, mairin and senkyunone were found to bind directly to ITGB3 and RORB proteins. Finally, mRNA and protein expression of ITGB3 and RORB was observed to be significantly down-regulated after incubation of MCF-7 cells with SWT. Overall, our results indicated that mairin and senkyunone were the key ingredients present in SWT, and ITGB3 as well as RORB proteins were the major targets affected by SWT. The prognostic model can be used to predict the outcome of BRCA patients.
基金：
National Natural Science Foundation of China [81673764]; Medical Science and Technology Research Fund project of Guangdong Province [A2022179]
第一作者机构：[1]Beijing Univ Chinese Med, Sch Life Sci, Beijing 100029, Peoples R China
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通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Zeye,Zhang Zexin,Song Jinqin,et al.Prognostic model construction and target identification of Si-Wu-Tang against breast cancer[J].HELIYON.2023,9(10):doi:10.1016/j.heliyon.2023.e20709.
APA：
Zhang, Zeye,Zhang, Zexin,Song, Jinqin,Wu, Wenfeng,Chen, Yiqi...&Zhao, Piwen.(2023).Prognostic model construction and target identification of Si-Wu-Tang against breast cancer.HELIYON,9,(10)
MLA：
Zhang, Zeye,et al."Prognostic model construction and target identification of Si-Wu-Tang against breast cancer".HELIYON 9..10(2023)
