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A Novel piRNA Associates with T2-high Asthma Phenotypes

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机构： [1]Clinical Big Data Research Center, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong, China [2]Channing Division of Network Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA [3]Shenzhen Key Laboratory of Chinese Medicine Active Substance Screening and Translational Research, Guangdong, Shenzhen, China [4]Computational Health Informatics Program, Boston Children’s Hospital, Boston, MA, USA [5]Division of Pulmonary Medicine, Department of Pediatrics, University of Pittsburgh, Pittsburgh, PA, USA [6]Partners Personalized Medicine, Partners Healthcare, Boston, MA, USA [7]Department of Pediatrics, Division of Respiratory Medicine, University of California San Diego, La Jolla, CA, USA

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关键词： piRNA T2-high asthma eosinophil IgE biomarker

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Piwi-interacting RNA (piRNA), the largest non-coding RNA group, regulate transcriptional processes. Whether piRNAs are associated with T2-high asthma is unknown.We sought to investigate the association between piRNAs and T2-high asthma in childhood asthma.We sequenced plasma samples from 462 subjects in the Childhood Asthma Management Program (CAMP) as the discovery cohort and 1,165 subjects in the Genetics of Asthma in Costa Rica Study (GACRS) as a replication cohort. Sequencing reads were filtered first and piRNA reads were annotated and normalized. Linear regression was used for the association analysis of piRNAs and peripheral blood eosinophil count, total serum IgE level and long-term asthma exacerbation in children with asthma. Mediation analysis was performed to investigate the effect direction. We then ascertained if the circulating piRNAs were present in asthmatic airway epithelial cells in a GEO public dataset.Fifteen piRNAs were significantly associated with eosinophil count in CAMP (p≤0.05), and three were successfully replicated in GACRS. Eleven piRNAs were associated with total IgE in CAMP, and one of these was replicated in GACRS. All 22 significant piRNAs were identified in epithelial cells in vitro, and six of these were differentially expressed between subjects with asthma and healthy controls. Fourteen piRNAs were associated with the long-term asthma exacerbation and effect of piRNAs on the long-term asthma exacerbation are mediated through eosinophils count and serum IgE level.piRNAs are associated with peripheral blood eosinophils and total serum IgE in childhood asthma and may play important roles in T2-high asthma.Copyright © 2023. Published by Elsevier Inc.

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大类 | 1 区

医学

小类 | 1 区过敏 1 区免疫学

最新[2025]版：

大类 | 1 区

医学

小类 | 1 区过敏 1 区免疫学

第一作者：

第一作者机构： [1]Clinical Big Data Research Center, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong, China [2]Channing Division of Network Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA [3]Shenzhen Key Laboratory of Chinese Medicine Active Substance Screening and Translational Research, Guangdong, Shenzhen, China

通讯作者：

通讯机构： [2]Channing Division of Network Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA [7]Department of Pediatrics, Division of Respiratory Medicine, University of California San Diego, La Jolla, CA, USA [*1]Department of Pediatrics, Division of Respiratory Medicine, University of California San Diego, La Jolla, CA, USA.

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A Novel piRNA Associates with T2-high Asthma Phenotypes

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