机构：[1]Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China广东省中医院[2]Department of National Institute of Stem Cell Clinical Research, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China, 广东省中医院[3]Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou, China,广东省中医院[4]Department of Shanghai Zhangjiang Biobank, National Engineering Centre for Biochip at Shanghai, Shanghai, China[5]Department of Dermatology, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China大德路总院皮肤科大德路总院皮肤科广东省中医院
The beneficial effect of quercetin in rheumatic diseases is unclear. Studies have already confirmed that human umbilical cord mesenchymal stem cells (hUCMSCs) alleviate some symptoms of rheumatoid arthritis (RA) by their immunosuppressive capacities. This study explored whether there are additive effects of quercetin and hUCMSCs on peripheral blood mononuclear cells (PBMCs) under simulated rheumatic conditions. hUCMSCs were pretreated with quercetin (10 mu M) before coculture with TNF-alpha/IFN-gamma-stimulated PBMCs at a ratio of 1:1 for 3 days. PBMC proliferation was inhibited, and the proportion of Th17 cells was shifted. These effects may be related to the effect of quercetin on functional molecules in hUCMSCs, including nitric oxide (NO), indoleamine 2,3-dioxygenase (IDO), interleukin 6 (IL-6) and Toll-like receptor-3 (TLR-3) and the Akt/I kappa B pathways. These results suggest that quercetin effectively promoted the immunoregulatory effect of hUCMSCs by inhibiting the Akt/I kappa B pathway, activating the Toll-like receptor-3 pathway, and regulating downstream cytokines.
基金：
Special Funding for TCM Science and Technology Research of Guangdong Provincial Hospital of Chinese Medicine [CMR-20170220-1001, YN2016QJ02]; Traditional Chinese Medicine Bureau Foundation of Guangdong Province [20183005]
第一作者机构：[1]Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China[2]Department of National Institute of Stem Cell Clinical Research, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China, [3]Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou, China,
通讯作者：
通讯机构：[1]Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China[2]Department of National Institute of Stem Cell Clinical Research, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China, [3]Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou, China,
推荐引用方式(GB/T 7714)：
Chen Guiling,Ye Yang,Cheng Ming,et al.Quercetin Combined With Human Umbilical Cord Mesenchymal Stem Cells Regulated Tumour Necrosis Factor-α/Interferon-γ-Stimulated Peripheral Blood Mononuclear Cells via Activation of Toll-Like Receptor 3 Signalling[J].FRONTIERS IN PHARMACOLOGY.2020,11:doi:10.3389/fphar.2020.00499.
APA：
Chen, Guiling,Ye, Yang,Cheng, Ming,Tao, Yi,Zhang, Kejun...&Huang, Yu.(2020).Quercetin Combined With Human Umbilical Cord Mesenchymal Stem Cells Regulated Tumour Necrosis Factor-α/Interferon-γ-Stimulated Peripheral Blood Mononuclear Cells via Activation of Toll-Like Receptor 3 Signalling.FRONTIERS IN PHARMACOLOGY,11,
MLA：
Chen, Guiling,et al."Quercetin Combined With Human Umbilical Cord Mesenchymal Stem Cells Regulated Tumour Necrosis Factor-α/Interferon-γ-Stimulated Peripheral Blood Mononuclear Cells via Activation of Toll-Like Receptor 3 Signalling".FRONTIERS IN PHARMACOLOGY 11.(2020)
