机构：[a]Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, 1023 South Shatai Road, Guangzhou, 510515, China[b]National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road,Beijing, 100101, China[c]Department of Liver Disease, Guangdong Hospital of Traditional Chinese Medicine (Zhuhai), 53 Jingle Road, Zhuhai, 519015, China大德路总院珠海院区外科大德路总院外一科广东省中医院[d]Center of Electron Microscopy, Central Laboratory, Southern Medical University, 1023 South Shatai Road, Guangzhou, 510515, China[e]State Key Laboratory of Biocontrol, School of Life sciences, Sun Yat-sen university, 135 Xingang Xi Road, Guangzhou, 510275, China[f]College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China[g]Department of TCM, Shenzhen Hospital of Southern Medical University, 1333 Xinhu Road, Shenzhen, 518101, China南方医科大学深圳医院深圳市康宁医院深圳医学信息中心
Hepatitis B virus (HBV) contains 3 types of particles, i.e., 22-nm-diameter spherical and tubular subviral particles (SVPs) and 44-nm-diameter Dane particles. The SVPs are non-infectious and present strong immunogenicity, while Dane particles are infectious. In this study, we isolated spherical SVPs from HBV carriers' sera and determined their 3D structure at the resolution of similar to 30 angstrom by cryo-electron microscopy (cryo-EM) single-particle reconstruction. Our cryo-EM structure suggests that the native HBV spherical SVP is irregularly organized, where spike-like features are arranged in a crystalline-like pattern on the surface. Strikingly, the hepatitis B surface antigen (HBsAg) in the native spherical SVPs folds as protrusions on the surface, as those on the native tubular SVPs and Dane particles, but is largely different from that in the recombinant octahedral SVPs. These results suggest a universal folding shape of HBsAg on the native HBV viral and subviral particles.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31425007, 31730023, 31521002]; National key research and development program of China [2017YFA0504700]; Strategic Priority Research Program from Chinese Academy of Sciences [XDB08010100]
第一作者机构：[a]Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, 1023 South Shatai Road, Guangzhou, 510515, China[b]National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road,Beijing, 100101, China
共同第一作者：
通讯作者：
通讯机构：[b]National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road,Beijing, 100101, China[f]College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China[*1]National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing, 100101, China
推荐引用方式(GB/T 7714)：
Jianhao Cao,Junchang Zhang,Yanmeng Lu,et al.Cryo-EM structure of native spherical subviral particles isolated from HBV carriers[J].VIRUS RESEARCH.2019,259:90-96.doi:10.1016/j.virusres.2018.10.015.
APA：
Jianhao Cao,Junchang Zhang,Yanmeng Lu,Shuhong Luo,Jingqiang Zhang&Ping Zhu.(2019).Cryo-EM structure of native spherical subviral particles isolated from HBV carriers.VIRUS RESEARCH,259,
MLA：
Jianhao Cao,et al."Cryo-EM structure of native spherical subviral particles isolated from HBV carriers".VIRUS RESEARCH 259.(2019):90-96
