机构：[1]Department of Breast, Longhua Hospital Affiliated to Shanghai University of TCM, Shanghai, 200032, China[2]Department of Mammary, Disease, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, Guangdong, 510120, China大德路总院乳腺科大德路总院乳腺科广东省中医院[3]Department of Breast Surgery, Shanghai Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, 200437, China
Background: Abnormally expressed long non-coding RNA (lncRNA) is associated with the development of breast cancer and multidrug resistance. However, the molecular mechanism by which lncRNA regulates cisplatin (DDP) in triple-negative breast cancer (TNBC) remains unclear. Methods: DDP resistant cell line MDA-MB-231/DDP was established by gradually increasing doses of DDP. Abnormal expression of lncRNA between MDA-MB-231 and MDA-MB-231/DDP was evaluated with microarray. In addition, cell proliferation was evaluated by CCK-8 and Ki67 assays. Furthermore, cell apoptosis was evaluated by cell apoptosis and TUNEL assays. Western blotting assay was used to detect the protein expression. In vivo animal study was performed finally. Results: HCP5 were significantly decreased in MDA-MB-231/DDP cells compared with MDA-MB-231 cells. Downregulation of HCP5 promoted DDP resistance in MDA-MB-231 cells by inhibiting PTEN expression. In contrast, overexpression of HCP5 reversed DDP resistance in MDA-MB-231/DDP cells by upregulating PTEN. In vivo experiments confirmed that overexpression of HCP5 inhibited DDP resistance in TNBC xenograft. Conclusion: We found that downregulation of HCP5 contributed to DDP resistance in TNBC, while overexpression of HCP5 reversed the resistance via upregulating PTEN level. Therefore, DDP combined with overexpression of HCP5 might be considered as a therapeutic approach for the treatment of DDP-resistant TNBC.
基金：
National Natural Science Fund for youth [81704074]; Longhua Medical Scholar Grant [LYTD-48]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区医学
小类|3 区医学：研究与实验3 区药学
最新[2025]版：
大类|2 区医学
小类|2 区医学：研究与实验2 区药学
JCR分区：
出版当年[2017]版：
Q2PHARMACOLOGY & PHARMACYQ2MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q1MEDICINE, RESEARCH & EXPERIMENTALQ1PHARMACOLOGY & PHARMACY
第一作者机构：[1]Department of Breast, Longhua Hospital Affiliated to Shanghai University of TCM, Shanghai, 200032, China
通讯作者：
通讯机构：[1]Department of Breast, Longhua Hospital Affiliated to Shanghai University of TCM, Shanghai, 200032, China[*1]Department of Breast, Longhua Hospital Affiliated to Shanghai University of TCM, No.725, Wanping South Road, Xuhui District, Shanghai, 200032, China.
推荐引用方式(GB/T 7714)：
Wu Jingjing,Chen Hao,Ye Meina,et al.Downregulation of long noncoding RNA HCP5 contributes to cisplatin resistance in human triple-negative breast cancer via regulation of PTEN expression[J].BIOMEDICINE & PHARMACOTHERAPY.2019,115:doi:10.1016/j.biopha.2019.108869.
APA：
Wu, Jingjing,Chen, Hao,Ye, Meina,Wang, Bing,Zhang, Yuzhu...&Chen, Hongfeng.(2019).Downregulation of long noncoding RNA HCP5 contributes to cisplatin resistance in human triple-negative breast cancer via regulation of PTEN expression.BIOMEDICINE & PHARMACOTHERAPY,115,
MLA：
Wu, Jingjing,et al."Downregulation of long noncoding RNA HCP5 contributes to cisplatin resistance in human triple-negative breast cancer via regulation of PTEN expression".BIOMEDICINE & PHARMACOTHERAPY 115.(2019)
