机构：[1]Department of Gastroenterology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Guangzhou, Guangdong 518001深圳市康宁医院深圳市人民医院深圳医学信息中心[2]Department of Gastroenterology, Kunshan Traditional Chinese Medicine Hospital, Suzhou, Jiangsu 215300[3]Department of Gastroenterology, PLA 411 Hospital, Shanghai 200081[4]Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433[5]Department of Hepatobiliary Surgery, The Second Hospital of Jiaxing, Jiaxing, Zhejiang 314000, P.R. China[6]Institute of Oncology, The Second Affiliated Hospital, Xi'an Medical College, 167 Fangdong Jie Road, Xi'an, Shaanxi 710038, P.R. China
Iodine-125 (I-125) seed radiation applied around the celiac ganglion can relieve the refractory pain in pancreatic cancer. In an in vitro cell radiation model of human neuroblastoma cell lines, the impact of I-125 radiation on the expression of transient receptor potential vanilloid-1 (TRPV1) was investigated. The results indicated that the radiation delivering doses <2.13 Gy did not significantly affect cell growth, whereas the doses >3.12 Gy significantly reduced cell viability. The reduced TRPV1 mRNA level was dependent on the doses, while the reduced protein level occurred at lower doses (2.63 and 4.27 Gy), then returned to normal at an intermediate dose of 5.09 Gy, and decreased again at higher doses (5.91 and 6.73 Gy). The miRNA profiling at the dose of 2.63 Gy revealed 32 and 22 miRNAs that were significantly upregulated and downregulated, respectively. In addition, the upregulated miR-1246 target, regulated the expression of TRPV1, indicating that miR-1246 may be a new therapeutic target for pancreatic pain.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81372482, 81472279, 81272663]; Natural Science Foundation of ShanghaiNatural Science Foundation of Shanghai [13ZR1409300, SYJY201714]
第一作者机构：[1]Department of Gastroenterology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Guangzhou, Guangdong 518001
共同第一作者：
通讯作者：
通讯机构：[4]Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433[6]Institute of Oncology, The Second Affiliated Hospital, Xi'an Medical College, 167 Fangdong Jie Road, Xi'an, Shaanxi 710038, P.R. China[*1]Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, P.R. China
推荐引用方式(GB/T 7714)：
Zhang Dingguo,Xu Hongwei,Wang Yuqiong,et al.I-125 radiation downregulates TRPV1 expression through miR-1246 in neuroblastoma cells[J].ONCOLOGY REPORTS.2019,42(1):243-252.doi:10.3892/or.2019.7133.
APA：
Zhang, Dingguo,Xu, Hongwei,Wang, Yuqiong,Wang, Kaixua,Wang, Yuxin...&Li, Zhaoshen.(2019).I-125 radiation downregulates TRPV1 expression through miR-1246 in neuroblastoma cells.ONCOLOGY REPORTS,42,(1)
MLA：
Zhang, Dingguo,et al."I-125 radiation downregulates TRPV1 expression through miR-1246 in neuroblastoma cells".ONCOLOGY REPORTS 42..1(2019):243-252
