Vespae nidus has been used in traditional Chinese medicine for thousands of years to treat a variety of diseases, including malignant tumors, rheumatoid arthritis, lung diseases, skin disease, digestive and urinary disorders, and dental diseases. As the same, Epimedii folium used to treat kidney-Yang deficiency,impotence and seminal emission, limp wilting sinews and bones, rheumatic arthralgia, Numbness and paralysis, osteoporosis. Vespae nidus-Epimedii folium formula used to treat chronic arthralgia due to Yang deficiency, and painful joints. But the underlying mechanisms by which it exerts therapeutic function have not been thoroughly studied. Cellular damage induced by free-radicals like reactive oxygen species has been implicated in several diseases. Hence naturally anti-oxidant play a vital role in combating these conditions. In this study, The effects of Vespae Nidus nidus-Epimedii folium formula aqueous extract (VEF) on free radical scavenging and anti-oxidative capacity is investigated. By free radical scavenging assays, the IC50 value of VEF were 71.33, 54.07 mu g/mL with DPPH, ABTS assay respectively, and 0.161mg ferrous sulfate/1 mg VEF with FRAP assay. These results indicated that the antioxidant properties of VEF hold great potential used as an alternative to more toxic synthetic anti-oxidants as an additive in food, cosmetic and pharmaceutical preparations for the oxidative diseases treatment.