机构:[1]Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China大德路总院检验科大德路总院检验科广东省中医院[2]Department of Gastroenterology, People Hospital, Hubei University of Medicine, Shiyan, China
Bacteria are subjected to sub-minimal inhibitory concentrations (sub-MIC) of antibiotics in various niches where the low-dosage treatment plays a key role in antibiotic resistance selection. However, the mechanism of sub-MIC of antibiotics on the resistant gene transfer is largely unknown. Here, we used Escherichia colt SM10 lambda pir in which the RP4 plasmid was chromosomally-integrated as the donor strain, to investigate the effects of sub-MIC of Ciprofloxacin(Cip) or Levofloxacin(Lev) on conjugational transfer of mobilisable plasmid-pUCP24T from SM10 lambda pir to Pseudomonas aeruginosa. The results showed that the transfer frequency was significantly increased by treating E. colt with sub-MIC of Cip or Lev. To investigate the molecular mechanisms, complete transcriptome sequencing was performed. We found that the sub-MIC of Cip or Lev enhanced the expression of several genes on the RP4 plasmid, which was consistent with the conjugation efficiency. Moreover, the expression of genes associated with SOS response in donor SM10 lambda pir was increased, but had no correlation with conjugation efficiency. These findings suggested that sub-MIC of Cip or Lev may promote conjugational transfer by up-regulating the expression of conjugation associated genes via an SOS-independent mechanism.
基金:
National Natural Science Foundation of China [81672081]; Guangzhou Science Technology and Innovation Commission [201707010296]
第一作者机构:[1]Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
通讯作者:
通讯机构:[1]Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China[*1]Guangzhou Higher Education Mega Center, NeiHuan Xi Road 55, Panyu District, Guangzhou, 510006, China
推荐引用方式(GB/T 7714):
E Shun-Mei,Zeng Jian-Ming,Yuan Hui,et al.Sub-inhibitory concentrations of fluoroquinolones increase conjugation frequency[J].MICROBIAL PATHOGENESIS.2018,114:57-62.doi:10.1016/j.micpath.2017.11.036.
APA:
E, Shun-Mei,Zeng, Jian-Ming,Yuan, Hui,Lu, Yang,Cai, Ren-Xin&Chen, Cha.(2018).Sub-inhibitory concentrations of fluoroquinolones increase conjugation frequency.MICROBIAL PATHOGENESIS,114,
MLA:
E, Shun-Mei,et al."Sub-inhibitory concentrations of fluoroquinolones increase conjugation frequency".MICROBIAL PATHOGENESIS 114.(2018):57-62