Protein-rich extract of Musca domestica larvae alleviated metabolic disorder in STZ-induced type 2 diabetic rat model via hepatoprotective and pancreatic β-cell protective activities
机构：[1]Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou 510006, Guangdong, China[2]School of Basic Courses, Guangzhou 510006, Guangdong, China[3]Department of Biochemistry and Molecular Biology, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou 510006, Guangdong, China[4]Department of Laboratory Science, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong, China广东省中医院深圳市中医院深圳医学信息中心[5]Key Laboratory of Digital Quality Evaluation of Chinese Material Medica of ASTCM, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou 510006, Guangdong, China
The study was designed to explore the beneficial effect of Musca domestica larvae extract (MDLE) on a metabolic disorder using a diabetic rat model. Streptozotocin-induced diabetic rats were treated with or without MDLE. Blood glucose, insulin levels, lipid profiles, and oxidative stress markers were measured. The morphological changes in the pancreas and liver were determined, as well as insulin expression. The expression of glucose transporter 4 (GLUT4), phospho-adenosine monophosphate-activated protein kinase (p-AMPK)/total AMPK, superoxide dismutase 1 (SOD1), catalase (CAT), and peroxisome proliferator-activated receptor gamma (PPAR) were detected. Compared with untreated diabetic rats, MDLE-treated rats had decreased urine volume, food intake, and water intake, along with significantly lower levels of blood glucose, malondialdehyde (MDA), plasma triglycerides, low-density lipoprotein (LDL), and total cholesterol. MDLE-treated rats also had higher levels of SOD activity, high-density lipoprotein (HDL), and insulin. MDLE treatment partially restored the -cell population, improved the liver necrosis and islet cell damage, reversed the decreased expression of GLUT4, phospho-AMPK, SOD1, and CAT in the liver, skeletal muscle and pancreatic tissue, and also increased the expression of PPAR in the liver and adipose tissue in diabetic rats. In conclusion, the obtained results suggest that MDLE could possibly be used pharmacologically as an adjuvant for the treatment of diabetes.
基金：
National Natural Science Foundation of China (NSFC, 81401150;
NSFC, 81403129); and the Science and Technology Cooperation
Research Project of Chinese Medicinal Medicine
Industry, Jianhe, Guizhou, China (Bureau of Science and
Technology of Jianhe County No. 5).
第一作者机构：[1]Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou 510006, Guangdong, China[2]School of Basic Courses, Guangzhou 510006, Guangdong, China[3]Department of Biochemistry and Molecular Biology, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou 510006, Guangdong, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Mei Hanfang,Xu Jianhua,He Yinru,et al.Protein-rich extract of Musca domestica larvae alleviated metabolic disorder in STZ-induced type 2 diabetic rat model via hepatoprotective and pancreatic β-cell protective activities[J].JOURNAL OF BIOSCIENCES.2018,43(5):969-983.doi:10.1007/s12038-018-9804-z.
APA：
Mei, Hanfang,Xu, Jianhua,He, Yinru,Yang, Xia,Liu, Wenbin...&Zhu, Jiayong.(2018).Protein-rich extract of Musca domestica larvae alleviated metabolic disorder in STZ-induced type 2 diabetic rat model via hepatoprotective and pancreatic β-cell protective activities.JOURNAL OF BIOSCIENCES,43,(5)
MLA：
Mei, Hanfang,et al."Protein-rich extract of Musca domestica larvae alleviated metabolic disorder in STZ-induced type 2 diabetic rat model via hepatoprotective and pancreatic β-cell protective activities".JOURNAL OF BIOSCIENCES 43..5(2018):969-983
