机构：[a]Center Lab of Longhua Branch, Shenzhen People's Hospital, 2nd Clinical Medical College of Jinan University, Shenzhen 518120, China深圳市龙华区人民医院深圳市人民医院深圳医学信息中心[b]Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Zhanjiang 524023, China[c]Department of Infectious disease, Shenzhen People's Hospital, 2nd Clinical Medical College of Jinan University, Shenzhen 518120, Guangdong Province, China深圳市康宁医院深圳市人民医院深圳医学信息中心[d]Department of pathology (Longhua Branch), Shenzhen People's Hospital, 2nd Clinical Medical College of Jinan University, Shenzhen 518120, Guangdong Province, China深圳市康宁医院深圳市龙华区人民医院深圳市人民医院深圳医学信息中心[e]College of Life Science, Jiangsu Normal University, Xuzhou 221116, Jiangsu Province, China[f]Key Lab for New Drug Research of TCM and Shenzhen Branch, State R&D Centre for Viro-Biotech, Research Institute of Tsinghua University in Shenzhen, Shenzhen518057, Guangdong, People's Republic of China
Six previously undescribed naturally occurring meroterpenoids (2, 5-9) together with seven known meroterpenoids (1, 3, 4, 10-13) were isolated from the root plant of Arnebia euchroma. Their structures and absolute configurations were determined by extensive D-1 NMR, C-13 NMR) and 2D NMR (H-1 -H-1 COSY, DEPT, HMQC, HMBC, NOESY) spectroscopic methods, spectroscopy high resolution mass spectrometry, as well as DFT and MM2 force-field calculations. Meroterpenoids 1-13 were evaluated for their cytotoxicities against human liver cancer cell lines SMMC-7721, HepG2, QGY-7703 and HepG2/ADM. Meroterpenoid 5 exhibited the most potent activity with IC50 values of 6.40 +/- 0.51, 3.86 +/- 0.28, 3.43 +/- 0.27 and 11.31 +/- 0.67 mu M, respectively. Meroterpenoid 4 exhibited significant growth inhibitory effects against HepG2/ADM with IC50 at 18.77 +/- 1.23 mu M, and meroterpenoid 8 with IC50 at 5.41 +/- 0.51 and 6.18 +/- 0.47 mu M against HepG2 and QGY-7703, respectively. These were more potent than the positive drug, Cisplatin.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81503221, 81703939]; Guangdong Natural Science Fund [2017A030313659, 2014A030310365]; Shenzhen Basic Research Project [JCYJ20170307095556333, JCYJ20170413093108233, JCYJ20160427183814675]
第一作者机构：[a]Center Lab of Longhua Branch, Shenzhen People's Hospital, 2nd Clinical Medical College of Jinan University, Shenzhen 518120, China
共同第一作者：
通讯作者：
通讯机构：[a]Center Lab of Longhua Branch, Shenzhen People's Hospital, 2nd Clinical Medical College of Jinan University, Shenzhen 518120, China[b]Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Zhanjiang 524023, China[f]Key Lab for New Drug Research of TCM and Shenzhen Branch, State R&D Centre for Viro-Biotech, Research Institute of Tsinghua University in Shenzhen, Shenzhen518057, Guangdong, People's Republic of China
推荐引用方式(GB/T 7714)：
Yang Wang,Yuzhen Zhu,Lingyun Xiao,et al.Meroterpenoids isolated from Arnebia euchroma (Royle) Johnst. and their cytotoxic activity in human hepatocellular carcinoma cells[J].FITOTERAPIA.2018,131:236-244.doi:10.1016/j.fitote.2018.11.005.
APA：
Yang Wang,Yuzhen Zhu,Lingyun Xiao,Lanlan Ge,Xin Wu...&Xiaobin Zeng.(2018).Meroterpenoids isolated from Arnebia euchroma (Royle) Johnst. and their cytotoxic activity in human hepatocellular carcinoma cells.FITOTERAPIA,131,
MLA：
Yang Wang,et al."Meroterpenoids isolated from Arnebia euchroma (Royle) Johnst. and their cytotoxic activity in human hepatocellular carcinoma cells".FITOTERAPIA 131.(2018):236-244
