机构：[1]Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou, China.[2]College of Medicine, Henan Engineering Research Center of Funiu Mountain's Medicinal Resources Utilization and Molecular Medicine, Pingdingshan University, Pingdingshan, Henan, China.[3]Department of Pharmacy, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, China.
A series of C21 steroidal glycosides were isolated from the root bark of Periploca sepium, including a new compound, perisepiumoside A1 (1), and six known compounds (2-7). Their structures were elucidated by analysis of HR-ESI-MS, and 1D and 2D NMR spectroscopic data. All these compounds were tested for their NO production inhibitory activity in LPS-stimulated RAW 264.7 cells. Results showed that these C21 steroidal glycosides could remarkably inhibit NO production, particularly 1 and 2 with IC50 values of 30.81 ± 0.18 μM and 44.39 ± 0.21 μM, respectively. In addition, the cytotoxicity of these compounds was measured on A549, MCF-7, and HeLa cancer cell lines. Among them, compounds 1 and 7 displayed cytotoxicity against the A549 cell line with IC50 values of 28.41 ± 0.12 μM and 39.06 ± 0.05 μM, respectively.
基金：
This study was funded by Scientific Research Foundation for the introduction of talent of Pingdingshan University (PXYBSQD2022040).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类|3 区化学
小类|3 区应用化学4 区药物化学
最新[2025]版：
大类|3 区化学
小类|3 区应用化学4 区药物化学
第一作者：
第一作者机构：[1]Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Shu Zhi-Heng,Chen Miao,Li Yao,et al.C21 steroidal glycosides from the root bark of Periploca sepium and their NO production inhibitory and cytotoxic activity[J].Natural product research.2023,1-7.doi:10.1080/14786419.2023.2269591.
APA：
Shu Zhi-Heng,Chen Miao,Li Yao,Fan Cai-Lian,Chen Wei-Wu...&Dai Yi.(2023).C21 steroidal glycosides from the root bark of Periploca sepium and their NO production inhibitory and cytotoxic activity.Natural product research,,
MLA：
Shu Zhi-Heng,et al."C21 steroidal glycosides from the root bark of Periploca sepium and their NO production inhibitory and cytotoxic activity".Natural product research .(2023):1-7
