机构：[1]Guangzhou Institute of Respiratory Disease, State Key Laboratory ofRespiratory Diseases, National Center for clinical research, The First AffiliatedHospital, Guangzhou Medical University, 1 Kangda Road, Guangzhou 510230,China[2]Institute of Tropical Medicine, Guangzhou University of ChineseMedicine, 12 Airport Road, Guangzhou 510405, China[3]OtolaryngologicalDepartment, Guangdong Provincial Hospital of Chinese Medicine, TheSecond Clinical College of Guangzhou University of Chinese Medicine,Guangzhou, China广东省中医院[4]Faculty of Chinese Medicine, Macau University ofScience and Technology, Avenida da Universidade, Taipa, Macau SAR 999078,China[5]Science department, University of British Columbia, 2329 West Mall,Vancouver, BC V6T 1Z4, Canada
Background: Lianhuaqingwen Capsule (LH-C) is a traditional Chinese medicine (TCM) formula used to treat respiratory tract infectious diseases in Chinese. The aim of this study was to determine the antiviral activity of LH-C and its immunomodulatory effects on viral infection. Method: The in vitro cytotoxicity and antiviral activity of LH-C was determined by MTT and Plaque reduction assays. Time course study under single-cycle virus growth conditions were used to determine which stage of viral replication was blocked. The effect of LH-C on the nuclear export of the viral nucleoprotein was examined using an indirect immunofluorescence assay. The regulation to different signaling transduction events and cytokine/ chemokine expression of LH-C was evaluated using Western blotting and real-time RT-PCR. After virus inoculation, BALB/ c mice were administered with LH-C of different concentrations for 5 days. Body-weight, viral titers and lung pathology of the mice were measured, the level of inflammatory cytokines were also examined using real-time RT-PCR. Results: LH-C inhibited the proliferation of influenza viruses of various strain in vitro, with the 50% inhibitory concentration (IC50) ranging from 0.35 to 2 mg/ mL. LH-C blocked the early stages (0-2 h) of virus infection, it also suppressed virusinduced NF-kB activation and alleviated virus-induced gene expression of IL-6, IL-8, TNF-a, IP-10, and MCP-1 in a dose-dependent manner. LH-C treatment efficiently impaired the nuclear export of the viral RNP. A decrease of the viral titers in the lungs of mice were observed in groups administered with LH-C. The level of inflammatory cytokines were also decreased in the early stages of infection. Conclusions: LH-C, as a TCM prescription, exerts broad-spectrum effects on a series of influenza viruses, including the newly emerged H7N9, and particularly regulates the immune response of virus infection. Thus, LH-C might be a promising option for treating influenza virus infection.
基金：
Guangdong Provincial Department of Science and Technology [2013B051000085, 2013B020224006]; Guangdong Natural Science Program [S2012030006598]; Innovation Academic Team of the Guangzhou Education System [13C07]; Collaborative Innovation Major Projects of Guangzhou Health Care [201400000002]; Municipal Science and Technology Bureau Foundation of Guangzhou [2014Y2-00031]
第一作者机构：[1]Guangzhou Institute of Respiratory Disease, State Key Laboratory ofRespiratory Diseases, National Center for clinical research, The First AffiliatedHospital, Guangzhou Medical University, 1 Kangda Road, Guangzhou 510230,China[2]Institute of Tropical Medicine, Guangzhou University of ChineseMedicine, 12 Airport Road, Guangzhou 510405, China
共同第一作者：
通讯作者：
通讯机构：[1]Guangzhou Institute of Respiratory Disease, State Key Laboratory ofRespiratory Diseases, National Center for clinical research, The First AffiliatedHospital, Guangzhou Medical University, 1 Kangda Road, Guangzhou 510230,China[4]Faculty of Chinese Medicine, Macau University ofScience and Technology, Avenida da Universidade, Taipa, Macau SAR 999078,China
推荐引用方式(GB/T 7714)：
Yuewen Ding,Lijuan Zeng,Runfeng Li,et al.The Chinese prescription lianhuaqingwen capsule exerts anti-influenza activity through the inhibition of viral propagation and impacts immune function[J].BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE.2017,17:doi:10.1186/s12906-017-1585-7.
APA：
Yuewen Ding,Lijuan Zeng,Runfeng Li,Qiaoyan Chen,Beixian Zhou...&Fengxue Zhang.(2017).The Chinese prescription lianhuaqingwen capsule exerts anti-influenza activity through the inhibition of viral propagation and impacts immune function.BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,17,
MLA：
Yuewen Ding,et al."The Chinese prescription lianhuaqingwen capsule exerts anti-influenza activity through the inhibition of viral propagation and impacts immune function".BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE 17.(2017)
