Influenza remains one of the major epidemic diseases worldwide, and rapid virus replication and collateral lung tissue damage caused by excessive pro-inflammatory host immune cell responses lead to high mortality rates. Thus, novel therapeutic agents that control influenza A virus (IAV) propagation and attenuate excessive pro-inflammatory responses are needed. Polysaccharide extract from Radix isatidis, a traditional Chinese herbal medicine, exerted potent anti-IAV activity against human seasonal influenza viruses (H1N1 and H3N2) and avian influenza viruses (H6N2 and H9N2) in vitro. The polysaccharides also significantly reduced the expression of pro-inflammatory cytokines (IL-6) and chemokines (IP-10, MIG, and CCL-5) stimulated by A/PR/8/34 (H1N1) at a range of doses (7.5 mg/mL, 15 mg/mL, and 30 mg/mL); however, they were only effective against progeny virus at a high dose. Similar activity was detected against inflammation induced by avian influenza virus H9N2. The polysaccharides strongly inhibited the protein expression of TLR-3 induced by PR8, suggesting that they impair the upregulation of pro-inflammatory factors induced by IAV by inhibiting activation of the TLR-3 signaling pathway. The polysaccharide extract from Radix isatidis root therefore has the potential to be used as an adjunct to antiviral therapy for the treatment of IAV infection.
基金：
National Natural Science Foundation of China [U1201227, U1301224, 81403167]; Scientific Research Project of College Directly Under the Guangzhou Education Bureau, China [1201430183]; Science and Technology Cooperation Program of Guangdong, China [2013B051000085]
第一作者机构：[1]Guangzhou Med Univ, Guangzhou Inst Resp Dis, Affiliated Hosp 1, Natl Clin Res Ctr Resp Dis,State Key Lab Resp Dis, Guangzhou 510120, Guangdong, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Guangzhou Med Univ, Guangzhou Inst Resp Dis, Affiliated Hosp 1, Natl Clin Res Ctr Resp Dis,State Key Lab Resp Dis, Guangzhou 510120, Guangdong, Peoples R China[5]Macau Univ Sci & Technol, AvenidaWai Long, Taipa 519020, Macau, Peoples R China
推荐引用方式(GB/T 7714)：
Li Zhengtu,Li Li,Zhou Hongxia,et al.Radix isatidis Polysaccharides Inhibit Influenza a Virus and Influenza A Virus-Induced Inflammation via Suppression of Host TLR3 Signaling In Vitro[J].MOLECULES.2017,22(1):doi:10.3390/molecules22010116.
APA：
Li, Zhengtu,Li, Li,Zhou, Hongxia,Zeng, Lijuan,Chen, Tingting...&Yang, Zifeng.(2017).Radix isatidis Polysaccharides Inhibit Influenza a Virus and Influenza A Virus-Induced Inflammation via Suppression of Host TLR3 Signaling In Vitro.MOLECULES,22,(1)
MLA：
Li, Zhengtu,et al."Radix isatidis Polysaccharides Inhibit Influenza a Virus and Influenza A Virus-Induced Inflammation via Suppression of Host TLR3 Signaling In Vitro".MOLECULES 22..1(2017)
