机构：[1]California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California 90095-1770, United States[2]Department of Pathology, Guangdong Provincial Hospital of TCM, Guangzhou University of Chinese Medicine, Guangzhou, China大德路总院珠海院区病理科病理科大德路总院病理科广东省中医院[3]PacGenomics, Agoura Hills, California 91301, United States[4]Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan, 115[5]Institute of Chemistry, Academia Sinica, Taipei, Taiwan, 115[6]Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan 106[7]Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, California 90048, United States[8]Department of Obstetrics and Gynecology, University of California, Los Angeles, Los Angeles, California 90095, United States
Circulating fetal nucleated cells (CFNCs) in maternal blood offer an ideal source of fetal genomic DNA for noninvasive prenatal diagnostics (NIPD). We developed a class of nanoVelcro microchips to effectively enrich a subcategory of CFNCs, i.e., circulating trophoblasts (cTBs) from maternal blood, which can then be isolated with single-cell resolution by a laser capture microdissection (LCM) technique for downstream genetic testing. We first established a nanoimprinting fabrication process to prepare the LCM-compatible nanoVelcro substrates. Using an optimized cTB-capture condition and an immunocytochemistry protocol, we were able to" identify and isolate single cTBs (Hoechst+/CK7+/HLA-G+/CD45, 20 mu m > sizes > 12 mu m) on the imprinted nanoVelcro microchips. Three cTBs were polled to ensure reproducible whole genome amplification on the cTBderived DNA, "paving the way for cTB-based array comparative genomic hybridization (aCGH) and short tandem repeats analysis. Using maternal blood samples collected from expectant mothers carrying a single fetus, the cTB-derived aCGH data were able to detect fetal genders and chromosomal aberrations, which had been confirmed by standard clinical practice. Our results support the use of nanoVelcro microchips for cTB-based noninvasive prenatal genetic testing, which holds potential for further development toward future NIPD solution.
基金：
National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R33-CA174562]
第一作者机构：[1]California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California 90095-1770, United States
共同第一作者：
通讯作者：
通讯机构：[1]California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California 90095-1770, United States[6]Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan 106
推荐引用方式(GB/T 7714)：
Hou Shuang,Chen Jie-Fu,Song Min,et al.Imprinted NanoVelcro Microchips for Isolation and Characterization of Circulating Fetal Trophoblasts: Toward Noninvasive Prenatal Diagnostics[J].ACS NANO.2017,11(8):8167-8177.doi:10.1021/acsnano.7b03073.
APA：
Hou, Shuang,Chen, Jie-Fu,Song, Min,Zhu, Yazhen,Jan, Yu Jen...&Tseng, Hsian-Rong.(2017).Imprinted NanoVelcro Microchips for Isolation and Characterization of Circulating Fetal Trophoblasts: Toward Noninvasive Prenatal Diagnostics.ACS NANO,11,(8)
MLA：
Hou, Shuang,et al."Imprinted NanoVelcro Microchips for Isolation and Characterization of Circulating Fetal Trophoblasts: Toward Noninvasive Prenatal Diagnostics".ACS NANO 11..8(2017):8167-8177
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