机构：[1]Department of Urology, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050035, Hebei, China河北医科大学第四医院[2]Department of Nursing, Hebei University of Chinese Medicine, Shijiazhuang 050020, Hebei, China[3]State Engineering Laboratory of Medical Key Technologies Application of Synthetic Biology, Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, Guangdong, China深圳市第二人民医院深圳医学信息中心[4]Department of Urology, Peking University Shenzhen Hospital, Institute of Urology of Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, Guangdong, China北京大学深圳医院深圳市康宁医院深圳医学信息中心香港大学深圳医院[5]Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, China浙江大学医学院附属第一医院[6]Longgang District Central Hospital, Shenzhen 518116, Guangdong, China深圳市龙岗区中心医院深圳医学信息中心
Calcitriol, also known as 1,25-dihydroxyvitamin D3 (1,25(OH)(2)VD3), is a biologically active form of vitamin D and has a wide range of anticancer activity against various cancer cell lines. However, the mechanism of calcitriol remains to be further studied. In this study, the biological effect and epigenetic regulation of calcitriol on kidney cancer cells were investigated. Calcitriol can significantly inhibit cell proliferation of kidney cancer cell lines 786-O (P<0.05). Calcitriol also induced cell apoptosis and senescence of 786-O and ACHN (P<0.05). Calcitriol can increase the expression of histone demethylase JMJD3 and cell senescence marker p16INK4A (P<0.05). Knockdown of JMJD3 decreased p16INK4A upregulation after calcitriol treatment (P<0.05), and also reduced calcitriol-induced cell senescence (P<0.05). This study reveals a new mechanism of anticancer activity of calcitriol by showing that histone demethylase JMJD3 induced by calcitriol increases p16INK4A expression and cell senescence. Therefore, these results provide new strategy for treatment and prevention of kidney cancer.
基金：
Natural Science Foundation of Hebei ProvinceNatural Science Foundation of Hebei Province [H2014205082, H2016423023]
第一作者机构：[2]Department of Nursing, Hebei University of Chinese Medicine, Shijiazhuang 050020, Hebei, China
共同第一作者：
通讯作者：
通讯机构：[3]State Engineering Laboratory of Medical Key Technologies Application of Synthetic Biology, Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, Guangdong, China[4]Department of Urology, Peking University Shenzhen Hospital, Institute of Urology of Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, Guangdong, China
推荐引用方式(GB/T 7714)：
Shen Yongqing,Yu Dan,Qi Pan,et al.Calcitriol induces cell senescence of kidney cancer through JMJD3 mediated histone demethylation[J].ONCOTARGET.2017,8(59):100187-100195.doi:10.18632/oncotarget.22124.
APA：
Shen, Yongqing,Yu, Dan,Qi, Pan,Wang, Xuliang,Guo, Xiaoqiang&Zhang, Aili.(2017).Calcitriol induces cell senescence of kidney cancer through JMJD3 mediated histone demethylation.ONCOTARGET,8,(59)
MLA：
Shen, Yongqing,et al."Calcitriol induces cell senescence of kidney cancer through JMJD3 mediated histone demethylation".ONCOTARGET 8..59(2017):100187-100195
医学