机构：[1]National Key Unit of Clinical Research of TCM On Chronic Kidney Diseases, Key Unit of Kidney Diseases, Guangdong Provincial Hospital of ChineseMedicine, Guangzhou, P.R. China[2]Medical College of Jinan University, Guangzhou, P.R. China
Objective: To investigate the effects of grape seed proanthocyanidin extract (GSPE) on indoxyl sulfate-induced Human Umbilical Vein Endothelial Cells (HUVECs) injury in vitro and study its mechanism. Methods: HUVECs were incubated with indoxyl sulfate at concentrations in the range found in uremic patients. Then we determined the effect of indoxyl sulfate on endothelial phenotype, endothelial function, ROS (reactive oxygen species), cell apoptosis and mitochondrial function. In addition, we detected whether GSPE can suppress the injury of HUVECs induced by indoxyl sulfate and probe the mechanism underlying the protective effects of GSPE by analyzing mitochondrial dysfunction. Results: GSPE treatment significantly attenuated indoxyl sulfate-induced HVUECs injury in a dose- and time-dependent manner. GSPE-enhanced eNOS and VE-cadherin expression, inhibited intracellular ROS level and cell apoptosis, adjust mitochondrial membrane potential and reduced 8-hydroxy-desoxyguanosine (8-OHdG) level induced by indoxyl sulfate. Conclusion: These results suggest that GSPE prevents HUVECs from indoxyl sulfate-induced injury by ameliorating mitochondrial dysfunction and may be a promising agent for treating uremia toxin-induced injury.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81202819]
第一作者机构：[1]National Key Unit of Clinical Research of TCM On Chronic Kidney Diseases, Key Unit of Kidney Diseases, Guangdong Provincial Hospital of ChineseMedicine, Guangzhou, P.R. China
共同第一作者：
通讯作者：
通讯机构：[1]National Key Unit of Clinical Research of TCM On Chronic Kidney Diseases, Key Unit of Kidney Diseases, Guangdong Provincial Hospital of ChineseMedicine, Guangzhou, P.R. China[*1]Key Unit of Kidney Diseases, Guangdong Provincial Hospital of Chinese Medicine 111th Dade Road, Guangzhou 510120, P.R. China.
推荐引用方式(GB/T 7714)：
Lu Zhaoyu,Lu Fuhua,Zheng Yanqun,et al.Grape seed proanthocyanidin extract protects human umbilical vein endothelial cells from indoxyl sulfate-induced injury via ameliorating mitochondrial dysfunction[J].RENAL FAILURE.2016,38(1):100-108.doi:10.3109/0886022X.2015.1104609.
APA：
Lu, Zhaoyu,Lu, Fuhua,Zheng, Yanqun,Zeng, Yuqun,Zou, Chuan&Liu, Xusheng.(2016).Grape seed proanthocyanidin extract protects human umbilical vein endothelial cells from indoxyl sulfate-induced injury via ameliorating mitochondrial dysfunction.RENAL FAILURE,38,(1)
MLA：
Lu, Zhaoyu,et al."Grape seed proanthocyanidin extract protects human umbilical vein endothelial cells from indoxyl sulfate-induced injury via ameliorating mitochondrial dysfunction".RENAL FAILURE 38..1(2016):100-108
