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Comparison of 5-hydroxytryptophan signaling pathway characteristics in diarrhea-predominant irritable bowel syndrome and ulcerative colitis

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机构: [1]Traditional Chinese Medicine Institute, Guangdong Medical University, Dongguan 524023, Guangdong Province, China [2]University City Branch Hospital of Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, Guangdong Province, China [3]Guangdong Key Laboratory of Natural Medicine Research and Development, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China [4]University City Branch Hospital of Guangdong Provincial Hospital of Traditional Chinese Medicine, Inner Ring Road, Panyu District, Guangzhou 510006, Guangdong Province, China.
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关键词: Stomach type diarrhea Diarrhea-predominant irritable bowel syndrome Ulcerative colitis in remission 5-Hydroxytryptophan

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AIM: To study differences in the visceral sensitivity of the colonic mucosa between patients with diarrhea-predominant irritable bowel syndrome (IBS-D) and those with ulcerative colitis (UC) in remission and to relate these differences with changes in the 5-hydroxytryptophan (5-HT) signaling pathway. METHODS: Gastrointestinal symptoms were used to determine the clinical symptom scores and rectal visceral sensitivity of patients with IBS-D and patients with UC in remission. Blood levels of 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) were measured using an HPLC-electrochemical detection system. The levels of 5-HT 3 receptor (3R), 4R, and 7R mRNAs in colonic biopsy samples were detected using reverse transcription-polymerase chain reaction. The protein expression of TPH1 was analyzed by Western blot and immunohistochemistry. RESULTS: Abdominal pain or discomfort, stool frequency, and the scores of these symptoms in combination with gastrointestinal symptoms were higher in the IBS-D and UC groups than in the control groups. However, no significant differences were observed between the IBS-D and UC remission groups. With respect to rectal visceral sensitivity, the UC remission and IBS-D groups showed a decrease in the initial perception threshold, defecating threshold and pain threshold. However, these groups exhibited significantly increased anorectal relaxation pressure. Tests examining the main indicators of the 5-HT signaling pathway showed that the plasma 5-HT levels, 5-HIAA concentrations, TPH1 expression in the colonic mucosa, and 5-HT3R and 5-HT5R expression were increased in both the IBS-D and the UC remission groups; no increases were observed with respect to 5-HT7R expression. CONCLUSION: The IBS-D and UC groups showed similar clinical symptom scores, visceral sensitivity, and levels of serotonin signaling pathway indicators in the plasma and colonic mucosa. However, the pain threshold and 5-HT7R expression in the colonic mucosa were significantly different between these groups. The results reveal that (1) IBS-D and UC are related to visceral sensitivity pathogenesis and the clinical manifestations of these conditions and (2) the observed differences in visceral hypersensitivity are possibly due to differences in levels of the 5-HT7 receptor, a component of the 5-HT signaling pathway.

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出版当年[2015]版:
大类 | 3 区 医学
小类 | 4 区 胃肠肝病学
最新[2025]版:
大类 | 3 区 医学
小类 | 4 区 胃肠肝病学
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出版当年[2014]版:
Q3 GASTROENTEROLOGY & HEPATOLOGY
最新[2024]版:
Q1 GASTROENTEROLOGY & HEPATOLOGY

影响因子: 最新[2024版] 最新五年平均 出版当年[2014版] 出版当年五年平均 出版前一年[2013版] 出版后一年[2015版]

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第一作者机构: [1]Traditional Chinese Medicine Institute, Guangdong Medical University, Dongguan 524023, Guangdong Province, China
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通讯机构: [4]University City Branch Hospital of Guangdong Provincial Hospital of Traditional Chinese Medicine, Inner Ring Road, Panyu District, Guangzhou 510006, Guangdong Province, China.
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