机构：[1]Department of Developmental and Regenerative Biology,Jinan University, Guangzhou 510632, China.[2]Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China.[3]Harrison International Peace Hospital,Hengshui, Hebei, China.[4]Department of Reproductive Medicine,Guangdong Provincial Hospital of Chinese Medicine,Guangzhou, China.广东省中医院
In order to understand the role of the protein zona pellucida 2 in fertilization, an antibody against a central segment of the zona pellucida 2 peptide, segment 190-505 (Z2eH), was prepared. The influence of the antibody on sperm-zona interaction was tested using the sperm-egg binding assay. The effect of the antibody on fertility was evaluated by passive immunization with anti-Z2eH antibody. Immunohistochemical assay showed that an antibody from rabbit reacted specifically with the natural zona pellucida on mouse ovarian sections. Immunofluorescence assay showed that the antibody bound specifically to the zonae pellucidae of the ovulated oocytes and 2-cell embryos after passive immunization. The antibody-treated oocytes bound capacitated sperm as control oocytes, passive immunization did not impede the action of sperm to fertilize the oocyte in vivo. These findings suggest that the central peptide of ZP2 (190-505) is immunogenic and contains zona pellucida-specific epitopes, however the central polypeptide might not be the crucial part from which to construct a functional domain to bind sperm.
基金：
Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2012DFH30060]
第一作者机构：[1]Department of Developmental and Regenerative Biology,Jinan University, Guangzhou 510632, China.[*1]Department of Developmental and Regenerative Biology, Jinan University, Guangzhou 510632, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Developmental and Regenerative Biology,Jinan University, Guangzhou 510632, China.[*1]Department of Developmental and Regenerative Biology, Jinan University, Guangzhou 510632, China
推荐引用方式(GB/T 7714)：
Cao Zuowu,Nie Chuncheng,Xie Yan,et al.The bioactivities of the central segment of Zp2 polypeptide[J].ZYGOTE.2016,24(5):768-774.doi:10.1017/S0967199416000095.
APA：
Cao, Zuowu,Nie, Chuncheng,Xie, Yan&Cai, Dongqin.(2016).The bioactivities of the central segment of Zp2 polypeptide.ZYGOTE,24,(5)
MLA：
Cao, Zuowu,et al."The bioactivities of the central segment of Zp2 polypeptide".ZYGOTE 24..5(2016):768-774
