机构：[1]College of Chinese Medicine, Hebei University, Baoding 071002, China[2]The School of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Province, China[3]Guangdong Provincial Academy of Chinese Medical Sciences, Guangdong Provincial Traditional Chinese Medicine Hospital, Guangzhou 510006,Guangdong Province, China广东省中医院
There is growing interest in reducing Bacille Calmette-Guerin (BCG) side effects while keeping intact its therapeutic efficacy. In the present study, we evaluated the efficacy of Sclerotia of Polyporus umbellatus FRIES (Zhuling) and its main ingredient Polyporus Polysaccharide (PPS) to attenuate side effects of BCG therapy in vivo. The results show that bladder cancer development in model rats exhibited significantly reduced cancer invasiveness with Zhuling PPS combined with BCG. Flow cytometric (FCM) analysis showed expression of costimulatory molecules CD86, CD40, and TLR4/CD14 significantly increased with Zhuling PPS in combination with BCG. Similarly, immunohistochemical analysis revealed stronger CD86 and CD40 staining. Our findings show Zhuling PPS strongly reduced side effects and displayed synergistic effects during BCG instillation in rat bladder cancer models. The findings also suggest that the attenuation effect may result from direct activation of dendritic cell (DC) TLR4. (C) 2014 Elsevier Ltd. All rights reserved.
基金：
National Natural Science Foundation of Hebei (No.H2013201222), Hebei Administration of Traditional ChineseMedince (Nos. 2013185, 2011106); Medical Science Foundationof Hebei University (No. 2012A2001), National Natural ScienceFoundation of Shanxi (2014021037-7).
第一作者机构：[1]College of Chinese Medicine, Hebei University, Baoding 071002, China[*1]Hebei University, College of Chinese Medicine,Number 342 Road, Nanshi District, Baoding 071002, China
通讯作者：
通讯机构：[1]College of Chinese Medicine, Hebei University, Baoding 071002, China[3]Guangdong Provincial Academy of Chinese Medical Sciences, Guangdong Provincial Traditional Chinese Medicine Hospital, Guangzhou 510006,Guangdong Province, China[*1]Hebei University, College of Chinese Medicine,Number 342 Road, Nanshi District, Baoding 071002, China
推荐引用方式(GB/T 7714)：
Zhang Guo-wei,Qin Gui-fang,Han Bo,et al.Efficacy of Zhuling polyporus polysaccharide with BCG to inhibit bladder carcinoma[J].CARBOHYDRATE POLYMERS.2015,118:30-35.doi:10.1016/j.carbpol.2014.11.012.
APA：
Zhang, Guo-wei,Qin, Gui-fang,Han, Bo,Li, Cai-xia,Yang, Hong-Gai...&Zeng, Xing.(2015).Efficacy of Zhuling polyporus polysaccharide with BCG to inhibit bladder carcinoma.CARBOHYDRATE POLYMERS,118,
MLA：
Zhang, Guo-wei,et al."Efficacy of Zhuling polyporus polysaccharide with BCG to inhibit bladder carcinoma".CARBOHYDRATE POLYMERS 118.(2015):30-35
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