In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received: a routine medium group (cultured in DMEM), a PLLA microsphere group (DMEM containing PLLA microspheres alone) and a PLLA/bpV(pic) group [DMEM containing PLLA/bpV(pic) microspheres]. The effects of PLLA/bpV(pic) microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV(pic) granulation rate was (88.2 +/- 5.6)%; particle size was (16.8 +/- 3.1)%, drug loading was (4.05 +/- 0.3)%; encapsulation efficiency was (48.5 +/- 1.8)%. The release time lasted for 30 days. In PLLA/bpV(pic) microsphere group, the cell survival rate was (95.2 +/- 4.77)%, and the length of dorsal root ganglion (DRG) was 718 +/- 95 mu m, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV(pic) microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG.
基金：
Science and Technology Planning Project of Guangdong Province, China [2011B031800205]
第一作者机构：[1]The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, China
通讯作者：
推荐引用方式(GB/T 7714)：
Lin Qiang,Chen Hai-yun,Li Hao-shen,et al.Preparation of PLLA/bpV(pic) microspheres and their effect on nerve cells[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES.2014,34(1):76-80.doi:10.1007/s11596-014-1234-z.
APA：
Lin, Qiang,Chen, Hai-yun,Li, Hao-shen&Cai, Yang-ting.(2014).Preparation of PLLA/bpV(pic) microspheres and their effect on nerve cells.JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES,34,(1)
MLA：
Lin, Qiang,et al."Preparation of PLLA/bpV(pic) microspheres and their effect on nerve cells".JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES 34..1(2014):76-80
