Identification of the bioactive components of orally administered Lithocarpus polystachyus Rehd and their metabolites in rats by liquid chromatography coupled to LTQ Orbitrap mass spectrometry
机构：[1]Second Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China[2]School of Chinese Pharmaceutical Science, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China
A rapid, sensitive and selective UPLC/MS method using LTQ Orbitrap mass spectrometry was established for the analysis and characterization of the main biological components and their metabolites in rat plasma, urine and feces following oral administration of Lithocarpus polystachyus extract. In vivo, 22 flavonoid metabolites were observed in rat plasma, and 13 metabolites were detected in rat urine, whereas just two aglycones of dihydrochalcone (3-hydroxy phloretin and phloretin) could be detected in rat feces. Among these metabolites, one new and a known dihydrochalcone metabolite were isolated and definitely identified. Besides, five dihydrochalcone metabolites were tentatively identified as new compounds. A metabolism study of 3-hydroxyphlorizin and phloridzin was also conducted. Glucuronidation was the main metabolic pathway of phloridzin, whereas glucuronidation and sulfonation were the main metabolic pathway of 3-hydroxyphlorizin. These results provided a basis for evaluating the bioactive components of a complex natural medicine and their mechanisms of actions. (C) 2014 Elsevier B.V. All rights reserved.
基金：
This work was supported by a project on the integration of Industry, Education and Research of Guangdong province (2012B091 100184) and the National Natural Science Foundation of China (No.81202888 and KAB112133K07).
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外文
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中科院(CAS)分区：
出版当年[2013]版：
大类|3 区医学
小类|3 区生化研究方法3 区分析化学
最新[2025]版：
大类|3 区医学
小类|3 区生化研究方法3 区分析化学
JCR分区：
出版当年[2012]版：
Q2BIOCHEMICAL RESEARCH METHODSQ2CHEMISTRY, ANALYTICAL
最新[2023]版：
Q2BIOCHEMICAL RESEARCH METHODSQ2CHEMISTRY, ANALYTICAL
第一作者机构：[1]Second Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China
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推荐引用方式(GB/T 7714)：
Li Xiong,Zhao Ya,Hou Shaozhen,et al.Identification of the bioactive components of orally administered Lithocarpus polystachyus Rehd and their metabolites in rats by liquid chromatography coupled to LTQ Orbitrap mass spectrometry[J].JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES.2014,962:37-43.doi:10.1016/j.jchromb.2014.05.016.
APA：
Li, Xiong,Zhao, Ya,Hou, Shaozhen,Huang, Song,Yang, Weiqin...&Zeng, Xing.(2014).Identification of the bioactive components of orally administered Lithocarpus polystachyus Rehd and their metabolites in rats by liquid chromatography coupled to LTQ Orbitrap mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,962,
MLA：
Li, Xiong,et al."Identification of the bioactive components of orally administered Lithocarpus polystachyus Rehd and their metabolites in rats by liquid chromatography coupled to LTQ Orbitrap mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 962.(2014):37-43
