机构：[a]Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, No. 74 Zhongshan Road II, Guangzhou 510089, China[b]Department of Digestive Diseases, the Second Affiliated Hospital of Sun Yat-sen University, No. 107 Yanjiang Road, Guangzhou 510020, China中山大学附属第二医院[c]Department of Cardiology, Guangdong Provincial Hospital of Traditional Chinese Medicine, No. 111 Dade Road, Guangzhou 510020, China大德路总院广东省中医院[d]Department of Respiratory Disease, the Second Affiliated Hospital of Sun Yat-sen University, No. 107 Yanjiang Road, Guangzhou 510020, China中山大学附属第二医院[e]Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892, USA
The nucleocapsid (N) protein is a structural component of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) and can induce antibody responses in SARS patients during infection. However, it is not known whether SARS-CoV N protein can induce a long persistence of memory T-cell response in human. In this study, we found that peripheral blood mononuclear cells (PBMCs) from fully recovered SARS individuals rapidly produced IFN-gamma and IL-2 following stimulation with a pool of overlapping peptides that cover the entire N protein sequence. The N-specific IFN-gamma(+)CD4(+) T cells were mainly composed of CD45RA(-)CCR7(+)CD62L(-) cells, whereas IFN-gamma(+)CD8(+) memory T cells were mostly contained within CD45RA(+)CCR7(-)CD62L(-) cell population. Epitope mapping study indicated that a cluster of overlapping peptides located in the C-terminal region (amino acids [aa] 331 to 362) of N protein contained at least two different T-cell epitopes. The results indicated that human memory T-cell responses specific for SARS-CoV N protein could persist for 2 years in the absence of antigen, which would be a valuable for the design of effective vaccines against SARS-CoV and for basic studies of human T-cell memory. (c) 2006 Elsevier Inc. All rights reserved.
基金：
National Nature
Science Foundation of China (No. 30340012), Scientific
Technology Program of Guangdong (No. 2003Z3-E0491),
Ministry of Education, Guangdong Province of China and the
National Key Basic Research Program of China (973) (No.
2001CB510007)
第一作者机构：[a]Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, No. 74 Zhongshan Road II, Guangzhou 510089, China
通讯作者：
推荐引用方式(GB/T 7714)：
Hui Peng,Li-tao Yang,Ling-yun Wang,et al.Long-lived memory T lymphocyte responses against SARS coronavirus nucleocapsid protein in SARS-recovered patients[J].VIROLOGY.2006,351(2):466-475.doi:10.1016/j.virol.2006.03.036.
APA：
Hui Peng,Li-tao Yang,Ling-yun Wang,Jian Li,Jun Huang...&Chang-you Wu.(2006).Long-lived memory T lymphocyte responses against SARS coronavirus nucleocapsid protein in SARS-recovered patients.VIROLOGY,351,(2)
MLA：
Hui Peng,et al."Long-lived memory T lymphocyte responses against SARS coronavirus nucleocapsid protein in SARS-recovered patients".VIROLOGY 351..2(2006):466-475
医学