To identify the different plant resources of Herba Rabdosiae Serrae by using Random Amplified Polymorphic DNA ( RAPD ) Analysis. The mini spin columns were used to extract the genomic DNA from five different plants of Herba Rabdosiae Serrae. With the DNA extracted from these plants as template,the 85 oligo nucleic acids (10 bp)as random primers,the polymer chain reaction (PCR) was done and the results were analysed by electro-pharoses. 12 primers were selected with polymorphism and 7 of them showed good polymorphism in RAPD map. RAPD method can be used to identify the plant resources of Herba Rabdosiae Serrae.
基金:
广东省科技厅粤港关键领域重点突破项目( 2009A030901011)
语种:
中文
第一作者:
第一作者机构:[1]广东省中药研究所,广东广州510520
通讯作者:
推荐引用方式(GB/T 7714):
莫小路,曾庆钱,黄珊珊,等.应用RAPD 技术对溪黄草基原植物分类鉴定[J].Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials.2012,35(9):