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应用RAPD 技术对溪黄草基原植物分类鉴定

[Identification of herba rabdosiae serrae from different plant resources by RAPD method].

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收录情况: ◇ 统计源期刊 ◇ 北大核心 ◇ CSCD-C

机构: [1]广东省中药研究所,广东广州510520
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关键词: 溪黄草 线纹香茶菜 RAPD 分子标记技术

摘要:
To identify the different plant resources of Herba Rabdosiae Serrae by using Random Amplified Polymorphic DNA ( RAPD ) Analysis. The mini spin columns were used to extract the genomic DNA from five different plants of Herba Rabdosiae Serrae. With the DNA extracted from these plants as template,the 85 oligo nucleic acids (10 bp)as random primers,the polymer chain reaction (PCR) was done and the results were analysed by electro-pharoses. 12 primers were selected with polymorphism and 7 of them showed good polymorphism in RAPD map. RAPD method can be used to identify the plant resources of Herba Rabdosiae Serrae.

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第一作者机构: [1]广东省中药研究所,广东广州510520
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