Berberine inhibits epithelial-mesenchymal transition and promotes apoptosis of tumour-associated fibroblast-induced colonic epithelial cells through regulation of TGF-β signalling.
机构：[1]Department of Traditional Chinese Medicine, Affiliated Bao’an Hospital of Shenzhen, Southern Medical University, Shenzhen 518000, Guangdong, China南方医科大学深圳医院深圳市康宁医院深圳医学信息中心[2]Department of Traditional Chinese Medicine, Affiliated Bao’an Hospital of Traditional Chinese Medicine of Shenzhen, Traditional Chinese Medicine University Of Guangzhou, Shenzhen 518000, Guangdong, China深圳市康宁医院深圳医学信息中心[3]Elemental Laboratory, Maternal and Child Health Hospital of Ganzhou, Zhangye 734000, Gansu, China
Tumour-associated fibroblasts (TAFs) mediate the differentiation of adjacent stromal cells. Berberine (BBR), a monomer of traditional Chinese herbs, exhibits a potent therapeutic effect against cancer. However, the effects of BBR on the differentiation of normal colonic epithelial cells induced by TAFs have not been determined. In the present study, we selected the TAF-like myofibroblast cell line CCD-18Co. CCD-18Co-derived conditioned medium (CM) and co-culture induced epithelial-mesenchymal transition (EMT) changes in colonic epithelial HCoEpiC cells with decreased E-cadherin and increased vimentin and α-SMA expression. In addition, CCD-18Co stimulated the expression of ZEB1 and Snail and promoted motility. We used LY364947, a TGF-β receptor kinase type I (TβRI) inhibitor, and BBR. Our results showed that LY364947 and BBR inhibited these phenomena. BBR decreased the expression of ZEB1 and Snail, and this effect was concentration dependent. BBR also downregulated the expression of TβRI, TβRII, Smad2/p-Smad2 and Smad3/p-Smad3. In addition, BBR induced apoptosis in EMT-like HCoEpiC cells in a concentration-dependent manner with upregulation of Bax and downregulation of Bcl-2. However, VX-702, an inhibitor of p38 MAPK, significantly suppressed the apoptosis rate. BBR promoted the expression of p38 MAPK and phosphorylated p38 MAPK. In conclusion, berberine inhibits EMT and promotes apoptosis in TAF-induced colonic epithelial cells through mediation of the Smad-dependent and SMAD-independent TGF-β signalling pathways.
基金：
Natural Science
Foundation of Guangdong Province (2018A030310060), China
Postdoctoral Foundation (2018 M643353).
第一作者机构：[1]Department of Traditional Chinese Medicine, Affiliated Bao’an Hospital of Shenzhen, Southern Medical University, Shenzhen 518000, Guangdong, China
通讯作者：
推荐引用方式(GB/T 7714)：
Huang Chao,Wang Xiu-Lian,Qi Fang-Fang,et al.Berberine inhibits epithelial-mesenchymal transition and promotes apoptosis of tumour-associated fibroblast-induced colonic epithelial cells through regulation of TGF-β signalling.[J].JOURNAL OF CELL COMMUNICATION AND SIGNALING.2020,14(1):53-66.doi:10.1007/s12079-019-00525-7.
APA：
Huang Chao,Wang Xiu-Lian,Qi Fang-Fang&Pang Zuo-Liang.(2020).Berberine inhibits epithelial-mesenchymal transition and promotes apoptosis of tumour-associated fibroblast-induced colonic epithelial cells through regulation of TGF-β signalling..JOURNAL OF CELL COMMUNICATION AND SIGNALING,14,(1)
MLA：
Huang Chao,et al."Berberine inhibits epithelial-mesenchymal transition and promotes apoptosis of tumour-associated fibroblast-induced colonic epithelial cells through regulation of TGF-β signalling.".JOURNAL OF CELL COMMUNICATION AND SIGNALING 14..1(2020):53-66
