Repetitive Transcranial Magnetic Stimulation Alleviates Neurological Deficits After Cerebral Ischemia Through Interaction Between RACK1 and BDNF exon IV by the Phosphorylation-Dependent Factor MeCP2.
机构：[1]Department of Neurology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, No. 13, Shiliugang Road, Guangzhou 510315, Guangdong Province, China[2]Department of Neurology, The Affiliated Changzhou No. 2 People’s Hospital of Nanjing Medical University, No. 29, Xinglong Alley, Changzhou 213003, Jiangsu Province, China
Repetitive transcranial magnetic stimulation (rTMS) is acknowledged as a form of neurostimulation, especially for functional recovery. The foundational knowledge of molecular mechanism is limited regarding its role in cerebral ischemia, for which the present study was designed. Primary neurons were treated with oxygen-glucose deprivation (OGD) and repetitive magnetic stimulation (rMS), in which brain-derived neurotrophic factor (BDNF) and transcription of BDNF exons were examined. Then, adenovirus vectors carrying siRACK1 sequence were delivered to primary neurons, followed by detection of the transcription of BDNF exons and the extent of methyl CpG binding protein 2 (MeCP2) phosphorylation. Results showed that BDNF and the transcription of BDNF exons were upregulated by rMS and OGD treatment, but decreased by extra treatment of RACK1 siRNA. Then, the mechanism investigations demonstrated that rMS increased the extent of MeCP2 phosphorylation to promote the interaction between RACK1 and BDNF exon IV. The aforementioned findings were further confirmed in vivo in middle cerebral artery occlusion (MCAO)-induced rat models, as indicated by improved neurological functions and reduced area of cerebral infarction. The study offers potential evidence for improvement of neurological deficits, highlighting the important role of rTMS for treatment of cerebral ischemia.
基金：
This work was funded by National Natural Science Foundation
of China (No.81671284 to XZ & No.81471338).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|2 区医学
小类|2 区临床神经病学2 区神经科学2 区药学
最新[2025]版：
大类|2 区医学
小类|1 区药学2 区临床神经病学2 区神经科学
第一作者：
第一作者机构：[1]Department of Neurology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, No. 13, Shiliugang Road, Guangzhou 510315, Guangdong Province, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, No. 13, Shiliugang Road, Guangzhou 510315, Guangdong Province, China[2]Department of Neurology, The Affiliated Changzhou No. 2 People’s Hospital of Nanjing Medical University, No. 29, Xinglong Alley, Changzhou 213003, Jiangsu Province, China
推荐引用方式(GB/T 7714)：
Hongzhan Li,Jianqing Shang,Chengliang Zhang,et al.Repetitive Transcranial Magnetic Stimulation Alleviates Neurological Deficits After Cerebral Ischemia Through Interaction Between RACK1 and BDNF exon IV by the Phosphorylation-Dependent Factor MeCP2.[J].Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics.2020,17(2):651-663.doi:10.1007/s13311-019-00771-y.
APA：
Hongzhan Li,Jianqing Shang,Chengliang Zhang,Rulan Lu,Junpao Chen&Xianju Zhou.(2020).Repetitive Transcranial Magnetic Stimulation Alleviates Neurological Deficits After Cerebral Ischemia Through Interaction Between RACK1 and BDNF exon IV by the Phosphorylation-Dependent Factor MeCP2..Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics,17,(2)
MLA：
Hongzhan Li,et al."Repetitive Transcranial Magnetic Stimulation Alleviates Neurological Deficits After Cerebral Ischemia Through Interaction Between RACK1 and BDNF exon IV by the Phosphorylation-Dependent Factor MeCP2.".Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 17..2(2020):651-663
：