机构：[1]Zhongnan Hospital, Wuhan University, 169 Donghu Rd, Wuhan 430071, Hubei, China[2]Department of Chemistry and Biochemistry, Jackson State University, 1400 Lynch Street, Jackson, MS 39217, USA[3]Key Laboratory of State Administration of Traditional Chinese Medicine, Sunshine Lake Pharma Co., Ltd, Dongguan 523850, Guangdong, China
A method based on microfluidic voltage-assisted liquid desorption electrospray ionization-tandem mass spectrometry (VAL-DESI-MS/MS) has been developed for fast quantification of free amino acids in food. Food extracts were transferred to the microfluidic platform and analyzed by liquid desorption ESI-MS/MS. Deuterated aspartic acid (i.e., 2,2,3-d3-Asp) was used as internal standard for analysis. The method had linear calibration curves with r2 values > 0.998. Limits of detection were at the level of sub μM for the amino acids tested, i.e., glutamic acid (Glu), arginine (Arg), tyrosine (Tyr), tryptophan (Trp), and phenylalanine (Phe). To validate the proposed method in food analysis, extracts of Cordyceps fungi were analyzed. Amino acid contents were found in the range from 0.63 mg/g (Tyr in Cordyceps sinensis) to 4.44 mg/g (Glu in Cordyceps militaris). Assay repeatability (RSD) was ≤ 5.2% for all the five amino acids measured in all the samples analyzed. Recovery was found in the range from 95.8 to 105.1% at two spiking concentrations of 0.250 mg/g and 1.00 mg/g. These results prove that the proposed microfluidic VAL-DESI-MS/MS method offers a quick and convenient means of quantifying free amino acids with accuracy and repeatability. Therefore, it may have potential in food analysis for nutritional and quality assessment purposes. Graphical abstract.
基金：
Zhongnan Hospital of
Wuhan University Science, Technology, and Innovation Seed Fund
(znpy2017023 and znpy2018116 to HH), Traditional Chinese Medicine
research project of Hubei Provincial Health Commission (ZY2019M033
to HH), and US National Institutes of Health (GM089557 to YML).
Hu Hankun,Smith Shila,Li Xiangtang,et al.Fast quantification of free amino acids in food by microfluidic voltage-assisted liquid desorption electrospray ionization-tandem mass spectrometry.[J].Analytical and bioanalytical chemistry.2020,412(8):1947-1954.doi:10.1007/s00216-020-02450-w.
APA：
Hu Hankun,Smith Shila,Li Xiangtang,Qian Zhengming,Su Yaxia...&Liu Yi-Ming.(2020).Fast quantification of free amino acids in food by microfluidic voltage-assisted liquid desorption electrospray ionization-tandem mass spectrometry..Analytical and bioanalytical chemistry,412,(8)
MLA：
Hu Hankun,et al."Fast quantification of free amino acids in food by microfluidic voltage-assisted liquid desorption electrospray ionization-tandem mass spectrometry.".Analytical and bioanalytical chemistry 412..8(2020):1947-1954
