机构：[1]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China[2]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China[3]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou University, Guangzhou 510120, Guangdong, China[4]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China[5]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China[6]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou University, Guangzhou 510120, Guangdong, China[7]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China[8]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China[9]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou University, Guangzhou 510120, Guangdong, China[10]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China
Due to its relatively small size, homology to humans, and susceptibility to human viruses, the tree shrew becomes an ideal alternative animal model for the study of human viral infectious diseases. However, there is still no report for the comprehensive glycan profile of the respiratory tract tissues in tree shrews. In this study, we characterized the structural diversity of N-glycans in the respiratory tract of tree shrews using our well-established TiO2-PGC chip-Q-TOF-MS method. As a result, a total of 219 N-glycans were identified. Moreover, each identified N-glycan was quantitated by a high sensitivity and accurate MRM method, in which 13C-labeled internal standards were used to correct the inherent run-to-run variation in MS detection. Our results showed that the N-glycan composition in the turbinate and lung was significantly different from the soft palate, trachea, and bronchus. Meanwhile, 28 high-level N-glycans in turbinate were speculated to be correlated with the infection of H1N1 virus A/California/04/2009. This study is the first to reveal the comprehensive glycomic profile of the respiratory tract of tree shrews. Our results also help to better understand the role of glycan receptors in human influenza infection and pathogenesis.
基金：
This research was funded by the Science and Technology
Development Fund, Macau SAR (file no. 023/2016/AFJ to
W.J.R.) and the National Natural Science Foundation of China
(81661168012 and 81471937). This work was also sponsored
by Guangzhou Medical University High-level University
Clinical Research and Cultivation Program (Guangzhou
Medical University released [2017] no. 160).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|2 区生物
小类|2 区生化研究方法
最新[2025]版：
大类|2 区生物学
小类|2 区生化研究方法
第一作者：
第一作者机构：[1]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China
共同第一作者：
通讯作者：
通讯机构：[9]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou University, Guangzhou 510120, Guangdong, China[10]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China[*1]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou University, Guangzhou 510120, Guangdong, China[*2]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China
推荐引用方式(GB/T 7714)：
Yau Lee-Fong,Chan Ka-Man,Yang Chun-Guang,et al.Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.[J].Journal of proteome research.2020,19(4):1470-1480.doi:10.1021/acs.jproteome.9b00727.
APA：
Yau Lee-Fong,Chan Ka-Man,Yang Chun-Guang,Ip Sun-Wai,Kang Yue...&Wang Jing-Rong.(2020).Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry..Journal of proteome research,19,(4)
MLA：
Yau Lee-Fong,et al."Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.".Journal of proteome research 19..4(2020):1470-1480
