机构：[1]Department of Hematology, Jiangmen Central Hospital, Jiangmen, China[2]Department of Hematology, General Hospital of Southern Theatre Command of PLA, Guangzhou, China[3]Translational Medicine Center, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China[4]Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development, Department of Biomedical Engineering, Jinan University, Guangzhou, China[5]Department of Hematology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China[6]The First School of Clinical Medicine, Southern Medical University, Guangzhou, China
Aplastic anemia (AA) is a bone marrow failure disease. It is difficult to treat AA and in addition, relapses are common because of its complex disease pathogenesis. Allogeneic bone marrow-derived mesenchymal stem cells (BMSCs) infusion is effective and safe treatment option for the AA patients. However, it found that BMSCs infusion in AA patients is less than 30% effective. Therefore, the key to improve the efficacy of BMSCs treatment in these patients is to enhance their homing efficiency to the target sites. Studies have shown that SDF-1/CXCR4 axis plays an important role in promoting BMSCs homing. In this study, human BMSCs were transduced with lentivirus stably expressing CXCR4-BMSCs. Transduced BMSCs resemble normal BMSCs in many ways. Migration ability of CXCR4-BMSCs towards SDF-1 was increased because of the overexpression of CXCR4. In the mice with bone marrow failure, the migration and colonization ability of CXCR4-BMSCs to the bone marrow was significantly improved as seen by IVIS imaging and FACS. The SDF-1 level in the bone marrow failure mice was significantly higher than the normal mice. Thus, from our study it is clear that after CXCR4-BMSCs were infused into mice with bone marrow failure, SDF-1 interacted with CXCR4 receptor, leading cells to migrate and colonize to bone marrow. Because of the high SDF-1 expression in mouse bone marrow and CXCR4 receptor expression in cells, BMSCs homing was increased.
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基金：
Key Project of National Natural Science
Foundation of China, Grant/Award Numbers:
81873426, 81903973
第一作者机构：[1]Department of Hematology, Jiangmen Central Hospital, Jiangmen, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Hematology, Jiangmen Central Hospital, Jiangmen, China[3]Translational Medicine Center, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China[5]Department of Hematology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China[*1]The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.[*2]Jiangmen Central Hospital, Jiangmen, China[*3]Translational Medicine Center, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
推荐引用方式(GB/T 7714)：
Lixuan Chen,Yonghua Li,Wancheng Chen,et al.Enhanced recruitment and hematopoietic reconstitution of bone marrow-derived mesenchymal stem cells in bone marrow failure by the SDF-1/CXCR4.[J].JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE.2020,14(9):1250-1260.doi:10.1002/term.3096.
APA：
Lixuan Chen,Yonghua Li,Wancheng Chen,Na Han,Keke Li...&Yang Xiao.(2020).Enhanced recruitment and hematopoietic reconstitution of bone marrow-derived mesenchymal stem cells in bone marrow failure by the SDF-1/CXCR4..JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,14,(9)
MLA：
Lixuan Chen,et al."Enhanced recruitment and hematopoietic reconstitution of bone marrow-derived mesenchymal stem cells in bone marrow failure by the SDF-1/CXCR4.".JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 14..9(2020):1250-1260
