机构：[1]Department of Internal Medicine of Traditional Chinese Medicine, College of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China[2]Department of Traditional Chinese Medicine, Medical College of China Three Gorges University, Yichang, Hubei 443002, China[3]Department of Rheumatology, Nanfang Hospital, Southern Medical University, No. 1838, North of Guangzhou Avenue, Guangzhou, Guangdong 510515, China[4]Laboratory Animal Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China[5]Department of Pathogen Biology, School of Public Health, Southern Medical University, Guangzhou, Guangdong 510515, China
The objective of the present study is to evaluate the inhibitory effects of taxol (PTX) on angiogenesis in a collagen-induced arthritis (CIA) mouse model. Collagen II (C II) and complete Freund's adjuvant (CFA) were used in C57BL/6 (H-2b) mice to generate the CIA model. Random grouping was performed in the normal control group, CIA model group, PTX 1.5 mg/kg group, PTX 1.0 mg/kg group, and PTX 0.5 mg/kg group. Arthritis index scores, tissue pathology scores, and synovium microvessel density (MVD) analysis were performed. Immunohistochemistry and enzyme-linked immunosorbent assay were used to detect the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-α (HIF-1α). The correlation between MVD and pathological scores and between MVD and the expression of VEGF as well as HIF-1α in the synovium were also evaluated. After PTX treatment, the three intervention group arthritis index scores were reduced when compared with the CIA group. The total histological scores in the three PTX treatment groups were lower than those in the CIA group. Similarly, PTX significantly alleviated the scores for synovitis, pannus formation, and bone destruction. Compared with the CIA group, the MVD of the three intervention groups decreased in a dose-dependent manner. The expression of VEGF and HIF-1α in synovial tissues and serum also significantly decreased after PTX treatment. Further analysis showed that MVD and pathological scores and MVD and expression of VEGF as well as HIF-1α in the synovium were positively correlated. PTX may alleviate CIA by suppressing angiogenesis, providing new insights into the treatment of rheumatoid arthritis (RA). VEGF and HIF-1α may be targets for PTX suppression of microvessel formation.
基金：
National Science
Foundation of China (81573730 and 81628017) and the Science and
Technology Planning Proje c t of Guangdong Province
(2016A020226047).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|4 区医学
小类|4 区风湿病学
最新[2025]版：
大类|3 区医学
小类|4 区风湿病学
第一作者：
第一作者机构：[1]Department of Internal Medicine of Traditional Chinese Medicine, College of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China
通讯作者：
通讯机构：[1]Department of Internal Medicine of Traditional Chinese Medicine, College of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China[3]Department of Rheumatology, Nanfang Hospital, Southern Medical University, No. 1838, North of Guangzhou Avenue, Guangzhou, Guangdong 510515, China
推荐引用方式(GB/T 7714)：
Xu Juan,Feng Zhitao,Chen Shixian,et al.Taxol alleviates collagen-induced arthritis in mice by inhibiting the formation of microvessels.[J].Clinical rheumatology.2019,38(1):19-27.doi:10.1007/s10067-017-3646-1.
APA：
Xu Juan,Feng Zhitao,Chen Shixian,Zhu Junqing,Wu Xianghui...&Li Juan.(2019).Taxol alleviates collagen-induced arthritis in mice by inhibiting the formation of microvessels..Clinical rheumatology,38,(1)
MLA：
Xu Juan,et al."Taxol alleviates collagen-induced arthritis in mice by inhibiting the formation of microvessels.".Clinical rheumatology 38..1(2019):19-27
