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Paris saponin VII reverses chemoresistance in breast MCF-7/ADR cells.

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机构： [1]School of Medicine, Zhejiang University, Hangzhou 310020, Zhejiang, PR China [2]Laboratory of Oncological Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou 646000, Sichuan, PR China [3]Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Department of Pharmacology, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, Shaanxi, PR China [4]Faculty of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shaanxi, PR China [5]Department of Pathology, No. 422 Hospital of PLA, Zhanjiang 524005, Guangdong, PR China

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关键词： Paris saponin VII (PSVII) Multidrug resistance (MDR) P-glycoprotein (P-gp)

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The development of a multidrug-resistant (MDR) phenotype is a main obstacle to the successful treatment of breast cancer. Saponins of several herbs are considered as promising candidates for drug resistance treatment. We extracted Paris saponin VII (PS VII) from Trillium tschonoskii Maxim. and investigated whether it could sensitize chemoresistant breast cancer cells MCF-7/ADR to the cytotoxic effects of adriamycin. MCF-7/ADR cells were exposed to 0.5 μM PSVII plus different concentrations of adriamycin (0-100 μM). Then, MTT assay and adriamycin accumulation assay were used to assess cell proliferation and intracellular adriamycin retention. P glycoprotein levels and intracellular rhodamine 123 (Rh-123) accumulations were investigated to measure the expression and activity of P-glycoprotein. A xenograft model of nude mouse was utilized to observe the effect of PSVII in vivo. Treatment with PSVII influenced cell viability of MCF-7/ADR cells, as well as sensitized MCF-7/ADR cells to the cytotoxic effects of adriamycin. Moreover, PSVII significantly downregulated MDR1 expression in MCF-7/ADR cells. Intravenous administration of PSVII significantly enhanced anticancer efficacy of adriamycin to MCF-7/ADR xenograft model in nude mice. These findings suggested a possible application of PSVII in combination with chemotherapy and/or as neo-adjuvant therapy in the treatment of MDR breast cancer. Copyright © 2018 Elsevier B.V. All rights reserved.

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出版当年[2018]版：

大类 | 3 区医学

小类 | 2 区全科医学与补充医学 2 区植物科学 3 区药物化学 3 区药学

最新[2025]版：

大类 | 2 区医学

小类 | 1 区全科医学与补充医学 1 区药学 2 区药物化学 2 区植物科学

第一作者：

第一作者机构： [1]School of Medicine, Zhejiang University, Hangzhou 310020, Zhejiang, PR China [2]Laboratory of Oncological Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou 646000, Sichuan, PR China

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通讯作者：

通讯机构： [1]School of Medicine, Zhejiang University, Hangzhou 310020, Zhejiang, PR China [2]Laboratory of Oncological Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou 646000, Sichuan, PR China [3]Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Department of Pharmacology, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, Shaanxi, PR China [*1]School of Medicine, Zhejiang University, Hangzhou 310020, Zhejiang, PR China [*2]Laboratory of Oncological Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou 646000, Sichuan, PR China.

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Paris saponin VII reverses chemoresistance in breast MCF-7/ADR cells.

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