机构：[1]Department of Pathophysiology, Institute of Brain Science Research, Key Laboratory of State Administration of Traditional Chinese Medicine of the People’s Republic of China, School of Medicine, Jinan University, Guangzhou 510632, Guangdong, China[2]Department of Pathology, Guangzhou Overseas Chinese Hospital, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong, China
Oxidative stress is a key factor of and closely implicated in the pathogenesis of Alzheimer's disease (AD). We herein used tert-butyl hydroperoxide (t-BHP) to induce oxidative stress and mimic oxidative neurotoxicity in vitro. Lycopene is a natural antioxidant that has a strong ability to eliminate free radicals and shows effective protection in some neurodegenerative disease models. However, the effect of lycopene on t-BHP-induced neuronal damage in primary mouse neurons is unknown. This study aimed to investigate the effects of lycopene on t-BHP-induced neuronal damage and the related mechanisms. We found that lycopene pretreatment effectively enhanced the cell viability, improved the neuron morphology, increased the GSH/GSSG level, restored the mitochondrial membrane potential (ΔΨm) and decreased reactive oxygen species generation. Furthermore, lycopene reduced the ratios of Bax:Bcl-2 and cleaved caspase-3:caspase-3 and the level of cytochrome C, increased the levels of synaptophysin (SYP) and postsynaptic density 95 (PSD95) and activated the PI3K/Akt pathway. In conclusion, lycopene attenuated oxidative stress and reduced t-BHP-induced cell apoptosis, and the mechanism is likely related to activation of the PI3K/Akt pathway. Therefore, lycopene is a potential agent for preventing oxidative stress-mediated AD.
基金：
Grants from
the National Natural Science Foundation of China (Nos. 81471236
and 81371442).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|4 区生物
小类|4 区生化与分子生物学
最新[2025]版：
大类|4 区生物学
小类|4 区生化与分子生物学
第一作者：
第一作者机构：[1]Department of Pathophysiology, Institute of Brain Science Research, Key Laboratory of State Administration of Traditional Chinese Medicine of the People’s Republic of China, School of Medicine, Jinan University, Guangzhou 510632, Guangdong, China
通讯作者：
推荐引用方式(GB/T 7714)：
Huang Cuiqin,Wen Caiyan,Yang Mei,et al.Lycopene protects against t-BHP-induced neuronal oxidative damage and apoptosis via activation of the PI3K/Akt pathway.[J].Molecular biology reports.2019,46(3):3387-3397.doi:10.1007/s11033-019-04801-y.
APA：
Huang Cuiqin,Wen Caiyan,Yang Mei,Gan Danhui,Fan Chongzhu...&Lu Daxiang.(2019).Lycopene protects against t-BHP-induced neuronal oxidative damage and apoptosis via activation of the PI3K/Akt pathway..Molecular biology reports,46,(3)
MLA：
Huang Cuiqin,et al."Lycopene protects against t-BHP-induced neuronal oxidative damage and apoptosis via activation of the PI3K/Akt pathway.".Molecular biology reports 46..3(2019):3387-3397
