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Improved approach for comprehensive profiling of gangliosides and sulfatides in rat brain tissues by using UHPLC-Q-TOF-MS.

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机构： [1]State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, China [2]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510120, China

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关键词： Acidic glycosphingolipids (GSLs) Gangliosides (GAs) Sulfatides (STs) Pre-fractionation Rat brain UHPLC-Q-TOF-MS

摘要：

Gangliosides (GAs) and sulfatides (STs) are major acidic glycosphingolipids (GSLs) that are particularly abundant in the central nervous system and associated with substantial neurodegenerative diseases. In this study, we developed an improved approach for the comprehensive profiling of GAs and STs in rat brain tissues by adopting a pre-fractionation step before the LC-MS analysis. The pre-fractionation step allows the efficient enrichment of different types of acidic GSLs and the removal of high-abundance interferences, thereby greatly enhanced the detection sensitivity and accuracy of low-abundance acidic GSLs. By using this improved approach, a total of 340 acidic GSLs (from 281 compositions) were characterized in rat brain tissues, including 277 GAs (from 230 compositions) and 63 STs (from 51 compositions), among which 57 GAs and 14 STs were novel acidic GSLs that have not been reported previously. This study represented the most comprehensive profiling of acidic GSLs in rat brain tissues. The result of this study greatly enlarged our understanding of the structural diversity of natural acidic GSLs, and provided important chemical information for the exploration of biological function of acidic GSLs in the central nervous system. Copyright © 2019 Elsevier B.V. All rights reserved.

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出版当年[2018]版：

大类 | 3 区生物

小类 | 3 区生化与分子生物学 3 区生物物理

最新[2025]版：

大类 | 3 区生物学

小类 | 3 区生物物理 4 区生化与分子生物学

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第一作者机构： [1]State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, China

通讯作者：

通讯机构： [1]State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, China [*1]State Key Laboratory of Quality Research in Chinese Medicine Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology Taipa, Macau, China.

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