机构：[1]Laboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China,[2]The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China,[3]Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou, China,广东省中医院[4]Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Traditional Chinese Medicine, Dongguan, China,深圳市中医院深圳医学信息中心[5]The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China,[6]Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, China,[7]Guangdong Longfan Biological Science and Technology Company, Ltd., Foshan, China
Ilex asprella is routinely used in China as a traditional medicinal herb to treat influenza (Flu). However, its specific antiviral activity and underlying molecular mechanism have not yet been determined. In this study, we sought to determine the antiviral activity and mechanism of Asprellcosides B, an active component extracted from Ilex asprella, and used against the influenza A virus cell culture. We also performed a computer-assisted structural modeling analysis and carried out surface plasmon resonance (SPR) experiments in the hope of determining the viral target of Asprellcosides B. Results from our studies show that Asprellcosides B reduced virus replication by up to 63% with an IC50 of about 9 μM. It also decreased the low pH-induced and virus-mediated hemolysis by 71% in vitro. Molecular docking simulation analysis suggested a possible binding of Asprellcosides B to the hemagglutinin (HA), which was confirmed by a surface plasmon resonance (SPR) assay. Altogether, our findings demonstrate that Asprellcosides B inhibits the influenza A virus, through a specific binding to the HA, resulting in the blockade of the HA-mediated membrane fusion.
基金：
This work was funded by the National Natural Science
Foundation of China (Nos. 81803813 and 81730061), the Major Program of Guangdong Natural Science Foundation of China
(Nos. S2012030006598 and S2012010011143), the Science &
Technology Planning Project of Guangdong Province of China
(No. 2013B020224005), the Science & Technology Planning
Project of Guangzhou (No. 201804010029), the Innovative
Program of State Key Laboratory of Virology (No. 2016IOV003),
Science & Technology Planning Project of Guangdong Province
Office of Education (No. 2014GKXM032), and the State Major
Research Program for Innovation Team of Guangdong Province
(No. E1-KFD015151K02).
第一作者机构：[1]Laboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China,
通讯作者：
通讯机构：[6]Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, China,[7]Guangdong Longfan Biological Science and Technology Company, Ltd., Foshan, China
推荐引用方式(GB/T 7714)：
Wen Zhang,Si-Tai Chen,Qiu-Yan He,et al.Asprellcosides B of Ilex asprella Inhibits Influenza A Virus Infection by Blocking the Hemagglutinin-Mediated Membrane Fusion[J].FRONTIERS IN MICROBIOLOGY.2019,9:doi:10.3389/fmicb.2018.03325.
APA：
Wen Zhang,Si-Tai Chen,Qiu-Yan He,Li-Quan Huang,Xiong Li...&Geng Li.(2019).Asprellcosides B of Ilex asprella Inhibits Influenza A Virus Infection by Blocking the Hemagglutinin-Mediated Membrane Fusion.FRONTIERS IN MICROBIOLOGY,9,
MLA：
Wen Zhang,et al."Asprellcosides B of Ilex asprella Inhibits Influenza A Virus Infection by Blocking the Hemagglutinin-Mediated Membrane Fusion".FRONTIERS IN MICROBIOLOGY 9.(2019)
