机构：[1]Department of Pain Management, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong Province, China[2]Department of Anesthesiology, Foshan Hospital of Traditional Chinese Medicine, Foshan, Guangdong Province, China[3]Department of Anesthesiology, The First People’s Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan, Guangdong Province, China[4]Department of Anesthesiology, The Sixth Affiliated Hospital of Guangzhou Medical University, Qinyuan, Guangdong Province, China
Neurotoxicity induced by the local anaesthetics has aroused concern. A previous study has shown that an overload of intracellular calcium was involved in the neurotoxic effect. Cav3.1 is one of the low-voltage-activated (LVA) calcium channels which play a key point to regulate the intracellular calcium ion level. This study aimed to investigate the changes of the Cav3.1 expression in the SH-SY5Y cells treated with lidocaine hydrochloride.
The SH-SY5Y cells were treated with different concentrations of lidocaine hydrochloride(1 mM, 5 mM and 10 mM, namely L1 group, L5 group and L10 group) and different exposure times (1 h,12 h and 24 h), respectively. Cell viability, Cav3.1 protein and mRNA expression were detected.
The results showed that cell viability decreased and Cav3.1 mRNA and protein expression increased with the concentration (from 1 mM to 10 mM) of the lidocaine hydrochloride and exposure time (from 1 h to 24 h) to the SH-SY5Y cell line increased.
Those data showed that lidocaine hydrochloride induced SH-SY5Y cell toxicity and up-regulated Cav3.1mRNA and protein expression.
基金：
National Natural Science Foundation of
China (Grant Nonos. 81471278 and 81100831) and the Medical Scientific
Research Projects of Foshan City, Guangdong Province (Grant Nono.
2014AB00275).
第一作者机构：[1]Department of Pain Management, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong Province, China[2]Department of Anesthesiology, Foshan Hospital of Traditional Chinese Medicine, Foshan, Guangdong Province, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Pain Management, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong Province, China[3]Department of Anesthesiology, The First People’s Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan, Guangdong Province, China[4]Department of Anesthesiology, The Sixth Affiliated Hospital of Guangzhou Medical University, Qinyuan, Guangdong Province, China[*1]Department of Anesthesiology, The Sixth Affiliated Hospital of Guangzhou Medical University, Qinyuan, Guangdong Province, China[*2]Department of Anesthesiology, The First People’s Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan, Guangdong Province, China[*3]Department of Pain Management, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong Province, China
推荐引用方式(GB/T 7714)：
Gong Qin,Wen Xianjie,Li Heng,et al.Up-regulation of Cav3.1 expression in SH-SY5Y cells induced by lidocaine hydrochloride.[J].ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY.2018,46:S372-S379.doi:10.1080/21691401.2018.1425697.
APA：
Gong Qin,Wen Xianjie,Li Heng,He Jian,Wang Yunhua...&Wang Xiaoping.(2018).Up-regulation of Cav3.1 expression in SH-SY5Y cells induced by lidocaine hydrochloride..ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,46,
MLA：
Gong Qin,et al."Up-regulation of Cav3.1 expression in SH-SY5Y cells induced by lidocaine hydrochloride.".ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY 46.(2018):S372-S379
