机构：[1]Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Guangzhou, China[2]Department of Neurobiology, Southern Medical University, Guangzhou, China[3]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China
Polycomb group (PcG) proteins regulate the epigenetic status of transcription regulatory states during development. Progression from pluripotency to differentiation requires the sequential activation and repression of different PcG target genes, however, the relationship between early patterning signals, PcG expression, and the development of the central nervous system is still unclear. Using various models of neuronal differentiation, we provide evidence that CBX2 is a negative regulator of neuronal differentiation. Knock-down of CBX2 expression promotes neurite development, while overexpression of CBX2 inhibits neurite development. Further, we found that CBX2 is a direct target gene of miR-124. During neuronal differentiation, CBX2 was decreased while miR-124 was increased. Mechanistically, CBX2 directly interacts with the promoter region of several neuro-associated genes and regulates their expression. We found that the neuron-specific GAP-43 gene could contribute to the stimulating effect on neurite development associated with inhibition of CBX2.
基金：
the China Postdoctoral Science
Foundation Funded Project (No. 2017M612695), Natural
Science Funds of China (Nos. 81171179 and 81272439),
Funds for Key Natural Science Foundation of Guangdong
(Nos. 2016B030230004 and S2013020012754), The Educational
Commission of Guangdong (No. 2013CXZDA008), Key
Projects of Health Collaborative Innovation of Guangzhou
(No. 201400000003-2) to XJ, also part of Fund from the
Guangdong Provincial Clinical Medical Centre for Neurosurgery
(No. 2013B020400005), National Natural Science Foundation
of China (No. 81071016), and the Program for New Century
Excellent Talents in University (No. NCET-12-0647).
第一作者机构：[1]Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Guangzhou, China
通讯作者：
推荐引用方式(GB/T 7714)：
Gu Xi,Wang Xuemin,Su Dazhuang,et al.CBX2 Inhibits Neurite Development by Regulating Neuron-Specific Genes Expression.[J].FRONTIERS IN MOLECULAR NEUROSCIENCE.2018,11:doi:10.3389/fnmol.2018.00046.
APA：
Gu Xi,Wang Xuemin,Su Dazhuang,Su Xiaohong,Lin Lifang...&Jiang Xiaodan.(2018).CBX2 Inhibits Neurite Development by Regulating Neuron-Specific Genes Expression..FRONTIERS IN MOLECULAR NEUROSCIENCE,11,
MLA：
Gu Xi,et al."CBX2 Inhibits Neurite Development by Regulating Neuron-Specific Genes Expression.".FRONTIERS IN MOLECULAR NEUROSCIENCE 11.(2018)
