Anticancer Activity of Phloretin Against Human Gastric Cancer Cell Lines Involves Apoptosis, Cell Cycle Arrest, and Inhibition of Cell Invasion and JNK Signalling Pathway.
机构：[1]Department of Oncology, Affiliated Nanhai Hospital of Southern Medical University Foshan, Oncology Section 2, Foshan, Guangdong, P.R. China[2]Department of Traditional Chinese Medicine, Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, P.R. China[3]Department of Gastroenterology, Feicheng Mining Central Hospital, Feicheng, Taian, Shandong, P.R. China
BACKGROUND Gastric cancer is one of most commonly diagnosed cancers and causes significant mortality worldwide. In this study, the antiproliferative and anticancer effects of Phloretin were evaluated against gastric cancer cell lines. MATERIAL AND METHODS MTT assay was used to assess the proliferation rate of gastric cancer cells. DAPI and annexin V/PI were used for detection of apoptotic cell death. Cell invasion was investigated by Transwell assays and the expression of the proteins was estimated by immunoblotting. RESULTS The results revealed that Phloretin exerts anticancer effects on all the gastric cancer cell lines used in this study. However, the anticancer effects were more significant (p<0.05) on the AGS cell line. Further, the effect of Phloretin on the viability of normal GES-1 cells was minimal. Apoptosis assays showed that Phloretin triggers apoptotic cell death in AGS gastric cancer cells. Phloretin could also cause the arrest of the AGS gastric cancer cells in the G2/M phase of the cell cycle and suppress their ability to migrate. Western blotting analysis revealed that Phloretin significantly decreased the expression of p-JNK and p-38. However, comparatively lower effects were observed on the expression of JNK and P38. CONCLUSIONS We showed that Phloretin is an important molecule for the treatment of gastric cancer.
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出版当年[2017]版：
大类|4 区医学
小类|4 区医学：研究与实验
最新[2025]版：
大类|4 区医学
小类|4 区医学：研究与实验
第一作者：
第一作者机构：[1]Department of Oncology, Affiliated Nanhai Hospital of Southern Medical University Foshan, Oncology Section 2, Foshan, Guangdong, P.R. China
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推荐引用方式(GB/T 7714)：
Min Xu,Weiguang Gu,Zhou Shen,et al.Anticancer Activity of Phloretin Against Human Gastric Cancer Cell Lines Involves Apoptosis, Cell Cycle Arrest, and Inhibition of Cell Invasion and JNK Signalling Pathway.[J].Medical science monitor : international medical journal of experimental and clinical research.2018,24:6551-6558.doi:10.12659/MSM.910542.
APA：
Min Xu,Weiguang Gu,Zhou Shen&Fang Wang.(2018).Anticancer Activity of Phloretin Against Human Gastric Cancer Cell Lines Involves Apoptosis, Cell Cycle Arrest, and Inhibition of Cell Invasion and JNK Signalling Pathway..Medical science monitor : international medical journal of experimental and clinical research,24,
MLA：
Min Xu,et al."Anticancer Activity of Phloretin Against Human Gastric Cancer Cell Lines Involves Apoptosis, Cell Cycle Arrest, and Inhibition of Cell Invasion and JNK Signalling Pathway.".Medical science monitor : international medical journal of experimental and clinical research 24.(2018):6551-6558
