机构：[1]College of Traditional Chinese Medicine of Jinan University, Institute of Integrated Traditional Chinese and Western Medicine of Jinan University,[2]The First Affiliated Hospital of Jinan University,[3]Department of Cellular Biology, Guangdong Province Key Lab of Bioengineering Medicine, Institute of Biomedicine, Jinan University, Guangdong, China
Triple negative breast cancer (TNBC) is a particular subtype of breast malignant tumor with poorer prognosis than other molecular subtypes. Currently, there is increasing focus on long non-coding RNAs (lncRNAs), which can act as competing endogenous RNAs (ceR-NAs) and suppress miRNA functions involved in post-transcriptional regulatory networks in the tumor. Therefore, to investigate specific mechanisms of TNBC carcinogenesis and improve treatment efficiency, we comprehensively integrated expression profiles, including data on mRNAs, lncRNAs and miRNAs obtained from 116 TNBC tissues and 11 normal tissues from The Cancer Genome Atlas. As a result, we selected the threshold with |log2FC|>2.0 and an adjusted p-value >0.05 to obtain the differentially expressed mRNAs, miRNAs and lncRNAs. Hereafter, weighted gene co-expression network analysis was performed to identify the expression characteristics of dysregulated genes. We obtained five co-expression modules and related clinical feature. By means of correlating gene modules with protein-protein interaction network analysis that had identified 22 hub mRNAs which could as hub target genes. Eleven key dysregulated differentially expressed micro RNAs (DEmiRNAs) were identified that were significantly associated with the 22 hub potential target genes. Moreover, we found that 14 key differentially expressed lncRNAs could interact with the key DEmiRNAs. Then, the ceRNA crosstalk network of TNBC was constructed by utilizing key lncRNAs, key miRNAs, and hub mRNAs in Cytoscape software. We analyzed and described the potential characteristics of biological function and pathological roles of the TNBC ceRNA co-regulatory network; also, the survival analysis was performed for each molecule. These findings revealed that ceRNA crosstalk network could play an important role in the development and progression for TNBC. In addition, we also identified that some molecules in the ceRNA network possess clinical correlation and prognosis.
基金：
National Natural Science Foundation of China (nos 81673979, 81473688, 81373314, 81173265); Education Program of China for New Century Excellent Talents (no NCET-13-0827); Traditional Chinese Medicine Administration Project of Guangdong Province, China (no 20141070); Science and Technology Program of Guangzhou, China (no 2014J4100104); Science and Technology Program of Guangdong, China (nos 2014A020212672, and 2014A020210015); Natural Science Foundation of Guangdong Province, China (nos 2016A030313114; 2015A030313333); National Undergraduate Training Programs for Innovation and Entrepreneurship in 2015 (no 201510559046); Guangzhou Municipal Enterprise Research and Development Institutions Construction Project (no 201503010064); The Fundamental Research Funds for the Central Universities\Research and Cultivation and Innovation Fund of Jinan University, Guangzhou, China (nos 21615412, 21615464, and 21617467); Guangzhou Municipal Science and Technology Program in 2018 (project leader: Min Ma).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区肿瘤学
最新[2025]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区肿瘤学
第一作者：
第一作者机构：[1]College of Traditional Chinese Medicine of Jinan University, Institute of Integrated Traditional Chinese and Western Medicine of Jinan University,
共同第一作者：
通讯作者：
通讯机构：[1]College of Traditional Chinese Medicine of Jinan University, Institute of Integrated Traditional Chinese and Western Medicine of Jinan University,[*1]College of Traditional Chinese Medicine of Jinan University, Institute of Integrated Traditional Chinese and Western Medicine of Jinan University, 601 Huangpu Ave. West, Guangzhou 510632, Guangdong, China
推荐引用方式(GB/T 7714)：
Yuan Naijun,Zhang Guijuan,Bie Fengjie,et al.Integrative analysis of lncRNAs and miRNAs with coding RNAs associated with ceRNA crosstalk network in triple negative breast cancer.[J].OncoTargets and therapy.2017,10:5883-5897.doi:10.2147/OTT.S149308.
APA：
Yuan Naijun,Zhang Guijuan,Bie Fengjie,Ma Min,Ma Yi...&Hao Xiaoqian.(2017).Integrative analysis of lncRNAs and miRNAs with coding RNAs associated with ceRNA crosstalk network in triple negative breast cancer..OncoTargets and therapy,10,
MLA：
Yuan Naijun,et al."Integrative analysis of lncRNAs and miRNAs with coding RNAs associated with ceRNA crosstalk network in triple negative breast cancer.".OncoTargets and therapy 10.(2017):5883-5897
