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Investigation of miRNA- and lncRNA-mediated competing endogenous RNA network in cholangiocarcinoma.

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机构: [1]Department of Surgery, The Second Affiliated Hospital of Zhengzhou University [2]Department of Urology,The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450014 [3]College of Traditional ChineseMedicine of Jinan University, Institute of Integrated Traditional Chinese and Western Medicine of Jinan University,Guangzhou, Guangdong 510632 [4]Department of Urology, The Second Affiliated Hospital of Zhengzhou University,Zhengzhou, Henan 450014 [5]The Nethersole School of Nursing, Faculty of Medicine, The Chinese University ofHong Kong, Hong Kong 999077 [6]Department of Gynecology and Obstetrics, The SecondAffiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China
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关键词: cholangiocarcinoma weighted gene co‑expression network analysis competing endogenous RNA network survival prognosis

摘要:
Cholangiocarcinoma (CCA) is a biliary malignancy which is prone to lymphatic metastasis and has a high mortality rate. This disease lacks effective therapeutic targets and prognostic molecular biomarkers. The aim of the current study was to investigate differentially expressed genes and elucidate their association with CCA and the underlying mechanisms of action. mRNAs, long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) obtained from 36 CCA samples and nine normal samples from The Cancer Genome Atlas were integrated. Subsequently, 1,095 differentially expressed (DE) mRNAs and 75 DE miRNAs were identified using a threshold of |log2 fold change|>2 and an adjusted P<0.01. Weighted gene co-expression network analysis was used to identify the DEmRNAs that could be key target genes in CCA. A total of 12 hub DEmRNAs were identified as targetable genes. Furthermore, the hub DEmRNAs-DElncRNAs pairs were identified using the miRTarBase and miRcode databases. Cytoscape software was used to construct and visualize the protein-protein interactions and the competing endogenous RNA network. Survival time analysis and correlation analysis were used to further evaluate the hub genes. The results obtained in the current study suggested that spalt like transcription factor 3 and OPCML intronic transcript 1 may serve an important role in the development and progression of CCA. Copyright: © He et al.

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出版当年[2018]版:
大类 | 4 区 医学
小类 | 4 区 肿瘤学
最新[2025]版:
大类 | 4 区 医学
小类 | 4 区 肿瘤学
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第一作者机构: [1]Department of Surgery, The Second Affiliated Hospital of Zhengzhou University
通讯作者:
通讯机构: [1]Department of Surgery, The Second Affiliated Hospital of Zhengzhou University [4]Department of Urology, The Second Affiliated Hospital of Zhengzhou University,Zhengzhou, Henan 450014 [*1]Department of Surgery, The Second Affiliated Hospital of Zhengzhou University, 2 Jingba Road, Zhengzhou, Henan 450014, P.R. China
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