机构：[1]Southern Medical University, Guangzhou[2]Department of Orthopedics, Nanshan District People's Hospital, Shenzhen, Guangdong深圳市康宁医院深圳市南山区人民医院深圳医学信息中心[3]Department of Orthopedics, The First Affiliated Hospital of Nanchang University[4]Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi[5]Department of Orthopedics, General Hospital of Guangzhou Military Command of PLA, Guangzhou, Guangdong, P.R. China
HELQ is a DNA helicase important for repair of DNA lesions and has been linked to several types of cancer. However, little is known about its relationship with osteosarcoma (OS) and its mechanism. In the present study, the expression of HELQ and its downstream mediators in OS cells was assayed by quantitative PCR and western blot analysis. The function of HELQ in OS cells was investigated by Transwell invasion, wound healing, CCK8 assays and Comet assay. The results demonstrated that HELQ gene and protein were expressed in OS cells. OS cell invasion, migration, proliferation and DNA damage repair were enhanced by HELQ knock-down with shRNA-lentivirus and inhibited by HELQ overexpression with lentivirus transfection. Furthermore, the antitumor activities of HELQ may be associated with upregulated expression of the DNA damage-related proteins CHK1 and RAD51. Our findings indicated that HELQ confers an anti-invasive phenotype on OS cells by activating the CHK1-RAD51 signaling pathway and suggested that HELQ could be recognized as a promising therapeutic target for OS and other types of malignant tumors.
基金：
The present study was supported by grants from the National
Natural Science Foundation of China (no. 81260400),
the Science and Technology Program of Shenzhen
(JCYJ20150402152130176), the Technology Research Development
and Creative Design Program of Shenzhen, Nanshan
District (2015002) and the Natural Science Foundation of
Jiangxi Province (no. 20142BAB205064).
第一作者机构：[1]Southern Medical University, Guangzhou[2]Department of Orthopedics, Nanshan District People's Hospital, Shenzhen, Guangdong
共同第一作者：
通讯作者：
通讯机构：[1]Southern Medical University, Guangzhou[3]Department of Orthopedics, The First Affiliated Hospital of Nanchang University[5]Department of Orthopedics, General Hospital of Guangzhou Military Command of PLA, Guangzhou, Guangdong, P.R. China[*1]Department of Orthopedics, The First Affiliated Hospital of Nanchang University, 17 Yong Wai Zheng Street, Nanchang, Jiangxi 330006, P.R. China[*2]Southern Medical University, 1023 Sha Tai Nan Road, Baiyun District, Guangzhou, Guangdong 510515, P.R. China
推荐引用方式(GB/T 7714)：
Dong Ning Liu,Yun Fei Zhou,Ai Fen Peng,et al.HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway.[J].ONCOLOGY REPORTS.2017,37(2):1107-1113.doi:10.3892/or.2016.5329.
APA：
Dong Ning Liu,Yun Fei Zhou,Ai Fen Peng,Xin Hua Long,Xuan Yin Chen...&Hong Xia.(2017).HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway..ONCOLOGY REPORTS,37,(2)
MLA：
Dong Ning Liu,et al."HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway.".ONCOLOGY REPORTS 37..2(2017):1107-1113
