机构：[1]School of Biomedical Technology, Guilin Medical University, No. 109, North 2nd Ring Road, Guilin 541004, Guangxi, China[2]School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, Guangdong, China[3]Affiliated Longhua Central Hospital, Guangdong Medical University, Shenzhen 541004, Guangdong, China[4]Tropical Medicine Institute, Guangzhou University of Chinese Medicine, No. 12, Jichang Road, Guangzhou 510405, Guangdong, China深圳市中医院深圳医学信息中心[5]Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, Guilin Medical University, Guilin 541004, Guangxi, China
A low-cost rodent model of HIV infection and which presents high application value is an effective tool to investigate HIV infection and pathogenesis. However, development of such a small animal model has been hampered by the unsuitability of rodent cells for HIV-1 replication given that the retrovirus HIV-1 has high selectivity to its host cell. Our study used the mouse leukemia cell lines L615 and L1210 that were induced by murine leukemia virus and transfected with hCD4/CCR5 loaded-lentiviral vector. Lentiviral vectors containing the genes hCD4/CCR5 under the transcriptional control of cytomegalovirus promoter were designed. Transfection efficiencies of human CD4 and CCR5 in L615 and L1210 cells were analyzed by quantitative real-time polymerase chain reaction (RT-PCR) and Western blot assay. Results showed that hCD4 and CCR5 proteins were expressed on the cell surface, demonstrating that the L615 and L1210 cells were humanized and that they possess the characteristics necessary for HIV infection of human host cells. Moreover, the sensitivity of human CD4/CCR5 transgenic mouse cells to HIV infection was confirmed by RT-PCR and ELISA. Mouse leukemia cell lines that could express hCD4 and CCR5 were thus established to facilitate normal entry of HIV-1 so that a human CD4/CCR5 transgenic mice cell model can be used to investigate the transmission and pathogenesis of HIV/AIDS and potential antiviral drugs against this disease.
基金：
This work was
supported by the National Natural Science Foundation of China (Nos.
81660755 and 81573861).
第一作者机构：[1]School of Biomedical Technology, Guilin Medical University, No. 109, North 2nd Ring Road, Guilin 541004, Guangxi, China
通讯作者：
通讯机构：[1]School of Biomedical Technology, Guilin Medical University, No. 109, North 2nd Ring Road, Guilin 541004, Guangxi, China[3]Affiliated Longhua Central Hospital, Guangdong Medical University, Shenzhen 541004, Guangdong, China
推荐引用方式(GB/T 7714)：
Ya-jing Li,Fu-yan ZhuGe,Chang-chun Zeng,et al.Establishment of mouse leukemia cell lines expressing human CD4/CCR5 using lentiviral vectors.[J].VIRUS GENES.2017,53(2):197-204.doi:10.1007/s11262-016-1423-x.
APA：
Ya-jing Li,Fu-yan ZhuGe,Chang-chun Zeng,Jin-yang He,Ning Tan&Juan Liang.(2017).Establishment of mouse leukemia cell lines expressing human CD4/CCR5 using lentiviral vectors..VIRUS GENES,53,(2)
MLA：
Ya-jing Li,et al."Establishment of mouse leukemia cell lines expressing human CD4/CCR5 using lentiviral vectors.".VIRUS GENES 53..2(2017):197-204
