机构：[1]Department of Rheumatology, TCM-Integrated Hospital, Southern Medical University, No. 13, Shiliugang Road, Haizhu District, Guangzhou 510315, Guangdong, People’s Republic of China [2]Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, People’s Republic of China [3]Department of Gastroenterology, Guangdong General Hospital and Guangdong Academy of Medical Sciences, South China University of Technology, Guangzhou 510080, People’s Republic of China 广东省人民医院[4]Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, People’s Republic of China [5]Undergraduate of Grade 2013, The First Clinical College, Southern Medical University, Guangzhou 510515, Guangdong, People’s Republic of China [6] Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA [7] Undergraduate of Grade 2013, School of Public Health, Southern Medical University, Guangzhou 510515, Guangdong, People’s Republic of China [8]Department of Gastroenterology, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an 223001, Jiangsu, People’s Republic of China [9]Department of Spleen and Stomach Diseases, Tai’an Hospital of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Tai’an 271000, Shandong, People’s Republic of China [10] Department of Thoracic Surgery, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou 510900, Guangdong, People’s Republic of China [11]Department of Traditional Chinese Medicine, The Second Clinical Medical College, Guangdong Medical University, Dongguan 523808, Guangdong, People’s Republic of China [12]Guangdong Key Laboratory for Research and Development of Natural Drug, Research Institute of Traditional Chinese Medicine, Guangdong Medical University, Zhanjiang 524023, Guangdong, People’s Republic of China
The CD8+CD28+/CD8+CD28- T lymphocyte balance is vital for human ulcerative colitis (UC) but has not been defined in experimental colitis. This investigation will try to identify the changes that occur in the CD8+CD28+/CD8+CD28- T lymphocyte balance during the progression of trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats.
The frequencies of blood CD8+CD28+ and CD8+CD28- T lymphocytes were detected in the rats belonging to the normal, model, and treated groups on five days using flow cytometry. The treated rats were administered with mesalazine and were euthanized after a 14-day treatment, as were the normal and model rats. The sensitivity and specificity of the CD8+CD28+/CD8+CD28- T lymphocyte balance in diagnosing early colitis were analyzed by receiver operating characteristics (ROC) curves. The frequencies of CD8+CD28+ and CD8+CD28- T lymphocytes in the colon tissue were tested via immunofluorescence. ELISA was used to measure the levels of the cytokines. Immunohistochemistry and Western blotting were used to detect the colonic expression of JAK3, STAT6, NFATc2, and GATA3.
We found that the ratio of CD8+CD28+/CD8+CD28- T lymphocytes decreased, as did the level of interleukin-7, but not IL-12p40, IL-13, or IL-15, in the blood; however, the ratio increased along with JAK3, STAT6, NFATc2, and GATA3 in the colon of the rats with colitis. The changes were effectively reversed through the administration of mesalazine for 13 days. Surprisingly, the balance in the blood could sensitively distinguish rats with early colitis from normal rats.
These data show that increase in CD8+CD28+ T cells in blood and decrease in CD8+CD28- T cells in colon are associated with experimental colitis.
基金：
the National
Natural Science Foundation of China (NSFC, No. 81300370) and by
the High Level Matching Research (with this NSFC Program, No.
2013003) of Nanfang Hospital, Southern Medical University (Institution
II, the competent department of this program). Shixue Dai is
the P.I. of this program and the Postdoctor of institution III. We
greatly thank Jianxin Diao and Yuanliang Liu, College of Traditional
Chinese Medicine, Southern Medical University, for their contribution
in cytometry, Western blotting, and immunohistochemical
staining. We also appreciate Xiaojuan Wu (now in the Department
of Laboratory Medicine, West China Hospital, Sichuan University) and Chunyan Wang, Department of Laboratory Medicine, Nanfang
Hospital, Southern Medical University, for their dedicated detecting
work on flow cytometry for CD8? T cells.
第一作者机构：[1]Department of Rheumatology, TCM-Integrated Hospital, Southern Medical University, No. 13, Shiliugang Road, Haizhu District, Guangzhou 510315, Guangdong, People’s Republic of China [2]Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, People’s Republic of China [3]Department of Gastroenterology, Guangdong General Hospital and Guangdong Academy of Medical Sciences, South China University of Technology, Guangzhou 510080, People’s Republic of China
通讯作者：
通讯机构：[1]Department of Rheumatology, TCM-Integrated Hospital, Southern Medical University, No. 13, Shiliugang Road, Haizhu District, Guangzhou 510315, Guangdong, People’s Republic of China [2]Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, People’s Republic of China [3]Department of Gastroenterology, Guangdong General Hospital and Guangdong Academy of Medical Sciences, South China University of Technology, Guangzhou 510080, People’s Republic of China
推荐引用方式(GB/T 7714)：
Shixue Dai,Hongxiang Gu,Qianyi Lin,et al.Disequilibrium in the CD8+CD28+/CD8+CD28- T Lymphocyte Balance Is Related to Prognosis in Rats with Trinitrobenzenesulfonic Acid-Induced Colitis.[J].DIGESTIVE DISEASES AND SCIENCES.2017,62(3):639-651.doi:10.1007/s10620-016-4424-7.
APA：
Shixue Dai,Hongxiang Gu,Qianyi Lin,Tiaosi Xing,Minhua Chen...&Yuzhen Zhu.(2017).Disequilibrium in the CD8+CD28+/CD8+CD28- T Lymphocyte Balance Is Related to Prognosis in Rats with Trinitrobenzenesulfonic Acid-Induced Colitis..DIGESTIVE DISEASES AND SCIENCES,62,(3)
MLA：
Shixue Dai,et al."Disequilibrium in the CD8+CD28+/CD8+CD28- T Lymphocyte Balance Is Related to Prognosis in Rats with Trinitrobenzenesulfonic Acid-Induced Colitis.".DIGESTIVE DISEASES AND SCIENCES 62..3(2017):639-651
