机构：[1]Vascular Biology Center, Department of Medicine, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, CB3303A, Augusta, GA, 30912, USA.[2]The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou Shi, Guangdong Sheng, China.[3]Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, People's Republic of China.[4]Department of Cardiology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People's Republic of China.
Transplanted induced pluripotent stem cells (IPSC ) and embryonic stem cells (ESC) exhibit enhanced survival in ischemic tissues and promote survival of neighboring cells via paracrine effects. Recent studies indicate that stem cells can secrete extracellular vesicles (EV), which can shuttle noncoding RNA between cells and facilitate intercellular signaling and communication between donor stem cells and recipient tissues. Direct transplantation of IPSC -derived EV (IPSC -EV) is highly effective at promoting survival and preventing apoptosis of cardiomyocytes in a mouse model of acute myocardial ischemia-reperfusion (MI/R). Here, we describe a feasible protocol to purify EV from cultured IPSC .
基金：
NIH grants AR070029 and HL086555
(to Y.T.), and HL076684 and HL62984 (to N.L.W.).
语种：
外文
PubmedID：
第一作者：
第一作者机构：[1]Vascular Biology Center, Department of Medicine, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, CB3303A, Augusta, GA, 30912, USA.[2]The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou Shi, Guangdong Sheng, China.
通讯作者：
通讯机构：[*1]Vascular Biology Center, Department of Medicine, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, CB3303A, Augusta, GA, 30912, USA.
推荐引用方式(GB/T 7714)：
Chen Zixin,Li Yongjun,Yu Hong,et al.Isolation of Extracellular Vesicles from Stem Cells.[J].Methods in molecular biology (Clifton, N.J.).2017,1660:389-394.doi:10.1007/978-1-4939-7253-1_32.
APA：
Chen Zixin,Li Yongjun,Yu Hong,Shen Yan,Ju Chengwei...&Tang Yaoliang.(2017).Isolation of Extracellular Vesicles from Stem Cells..Methods in molecular biology (Clifton, N.J.),1660,
MLA：
Chen Zixin,et al."Isolation of Extracellular Vesicles from Stem Cells.".Methods in molecular biology (Clifton, N.J.) 1660.(2017):389-394
