机构：[1]Department of Medical Oncology, Hainan Province Hospital of Traditional Chinese Medicine, Haikou, China.[2]Guangdong Provincial Key Laboratory of Cancer Immunotherapy, Cancer Research Institute, School of Basic Medical Sciences, Southern Medical Sciences, Southern Medical University, Guangzhou, China.[3]TCM-Integrated Cancer Center, Southern Medical University, Guangzhou, China.[4]Department of Medical Oncology, TCM-Integrated Cancer Center of Southern Medical University, Guangzhou, China.[5]Department Surgical Oncology, Haikou People’s Hospital, Haikou, China.[6]College of Traditional Chinese Medicine, Hainan Medical University, Haikou, Hainan, China.
Schizophrenic patients tend to have reduced incidence of some cancers due to the treatment of antipsychotic drugs with antitumor effects, such as chlorpromazine and trifluoperazine (TFP). Forkhead Box O1 (FOXO1) as tumor suppressor in many malignancies is often inactivated by nuclear export, which could be inhibited by TFP. However, the antitumor efficiency of TFP and related role of FOXO1 in hepatocellular carcinoma (HCC) are unclear. Thus, two HCC cell lines SMMC-7721 and Bel-7402 were treated with different concentrations of TFP and the IC50 was determined. We found that TFP could inhibit the vitality of two cell lines and induce cell cycle arrest at G0/G1. Meanwhile, the apoptosis was also increased and the ability of migration or invasion was found to be impaired by TFP. Interestingly, TFP reversed the cytoplasmic localization of FOXO1 to nuclear and increased its expression in nuclear, and increased the ratio of Bax/Bcl-2. However, knockdown of FOXO1 significantly abrogated the TFP-induced
apoptosis by decreasing the Bcl-2 expression [corrected]. Furthermore, we found that TFP in vivo could effectively restrict the angiogenesis and tumor growth with reduced expression of VEGF, Bcl-2, and PCNA, and increased the nuclear localization of FOXO1, which indicated its antitumor role in HCC.
基金：
This work was supported by a Grant from Social Development
Special Project of Science and Technology Department
of Hainan Province Key Research (Grant number
ZDYF2016123).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|3 区生物
小类|4 区生化与分子生物学4 区细胞生物学4 区遗传学
最新[2025]版：
大类|4 区生物学
小类|4 区生化与分子生物学4 区细胞生物学4 区遗传学
第一作者：
第一作者机构：[1]Department of Medical Oncology, Hainan Province Hospital of Traditional Chinese Medicine, Haikou, China.[*1]Department of Medical Oncology, Hainan Province Hospital of Traditional Chinese Medicine, No. 47 Heiping Bei Road, Meilan District, Haikou City 571199 Hainan Province, China
通讯作者：
通讯机构：[1]Department of Medical Oncology, Hainan Province Hospital of Traditional Chinese Medicine, Haikou, China.[*1]Department of Medical Oncology, Hainan Province Hospital of Traditional Chinese Medicine, No. 47 Heiping Bei Road, Meilan District, Haikou City 571199 Hainan Province, China
推荐引用方式(GB/T 7714)：
Jingwen Jiang,Zhongxi Huang,Xuewu Chen,et al.Trifluoperazine Activates FOXO1-Related Signals to Inhibit Tumor Growth in Hepatocellular Carcinoma.[J].DNA and cell biology.2017,36(10):813-821.doi:10.1089/dna.2017.3790.
APA：
Jingwen Jiang,Zhongxi Huang,Xuewu Chen,Rongcheng Luo,Hongbin Cai...&Yunfang Zhang.(2017).Trifluoperazine Activates FOXO1-Related Signals to Inhibit Tumor Growth in Hepatocellular Carcinoma..DNA and cell biology,36,(10)
MLA：
Jingwen Jiang,et al."Trifluoperazine Activates FOXO1-Related Signals to Inhibit Tumor Growth in Hepatocellular Carcinoma.".DNA and cell biology 36..10(2017):813-821
